基于ISSR与RAPD标记分析内蒙古赤芍的遗传多样性  

Genetic Diversity Analysis of Paeoniae Radix Rubra from Inner Mongolia Using ISSR and RAPD

作　　者：杜亚楠 张敏[1,2,3] 孙淑英 陈贵林[1,2,3] DU Ya'nan;ZHANG Min;SUN Shuying;CHEN Guilin(School of Life Sciences, Inner Mongolia University, Hohhot 010070, China;2The Good Agriculture Practice Engineering Technology Research Center of Chinese and Mongolian Medicine in Inner Mongolia, Hohhot 010070, China;3Key Laboratory of Herbage & Endemic Crop Biology, Ministry of Education, Hohhot 010070, China)

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010070 [2]内蒙古自治区中蒙药材规范化生产工程技术研究中心,呼和浩特010070 [3]牧草与特色作物生物学教育部重点实验室,呼和浩特010070

出　　处：《西北植物学报》2021年第6期952-961,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：内蒙古科技厅应用技术资金项目(201702114)。

摘　　要：采用ISSR和RAPD分子标记技术对28个赤芍种群进行遗传变异和亲缘关系分析,为准确地评价赤芍种质的遗传特征、资源保护及新品种选育提供理论依据。结果显示:(1)利用分别筛选的14条ISSR和RAPD引物扩增出257条和215条条带,其中多态性条带分别为251条和209条,多态性条带百分率分别为97.8%和97.2%;同时证实野生赤芍种群的遗传多样性高于栽培种群。(2)根据Shannon's信息指数(I)和Nei's基因多样性指数(He)值,发现内蒙古多伦种群(DL)的遗传多样性水平最高,建议在此地建立野生赤芍资源保护区。(3)根据遗传分化系数(Gst),发现野生赤芍种群的遗传分化主要发生在种群内,可能由遗传漂变引起;而栽培赤芍种群的遗传分化主要在种群间,说明栽培赤芍种群间的基因交流较少。(4)两种分子标记的聚类分析结果均将28个赤芍种群聚为5大类,遗传距离变化范围分别为0.1151~0.3438和0.0955~0.2862。研究表明,互相印证的ISSR和RAPD方法可以在DNA水平上更准确有效地分析赤芍种质资源的遗传结构和遗传多样性。We used ISSR and RAPD molecular markers to analyze the genetic variation and relationship of 28 Paeoniae Radix Rubra(PRR),which provides a biological foundation for accurately evaluating the genetic characteristics,protecting the germplasm resources and cultivating the excellent new varieties of PRR.The results showed that:(1)257 and 215 bands were amplified by 14 ISSR and 14 RAPD markers.The polymorphic bands and polymorphism ratio were found to be 251,209 and 97.8%,97.2%,respectively.Two molecular markers showed that the genetic diversity of wild PRR population was higher than that of cultivated population.(2)According to Shannon's information index(I)and Nei's gene diversity(He),the genetic diversity of population of Duolun County,Inner Mongolia(DL)was the highest,where can be recommended to establish a wild PRR nature reserve.(3)The genetic differentiation coefficient(Gst)indicated that the genetic differentiation of wild PRR populations was mainly within the population which may be caused by genetic drift.Conversely,the genetic differentiation of the cultivated PRR populations was mainly among the populations,indicating that there was less gene exchange among cultivated populations.(4)A UPGMA cluster grouped the 28 populations into 5 major clusters based on both ISSR and RAPD markers and their genetic distances were 0.1151-0.3438 and 0.0955-0.2862,respectively.This research shows that the complementary ISSR and RAPD methods can effectively analyze the genetic structure and genetic diversity of PRR populations at the DNA level.

关 键 词：赤芍 遗传多样性 ISSR RAPD 资源保护 

分 类 号：Q346.5[生物学—遗传学] Q789