肾组织内miR-29a下调促进叶酸诱导的急性肾损伤  

Down-regulation of miR-29a in renal tissue promotes folic acid-induced acute kidney injury

作　　者：王冬冬[1] 栾军军 张旖骁[2] 周华[1] WANG Dong-dong;LUAN Jun-jun;ZHANG Yi-xiao;ZHOU Hua(Department of Nephrology,The Affiliated Shengjing Hospital of China Medical University,Shenyang 110004,China;Department of Urology,The Affiliated Shengjing Hospital of China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院肾内科,辽宁沈阳110004 [2]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004

出　　处：《解剖科学进展》2021年第3期284-287,共4页Progress of Anatomical Sciences

基　　金：国家重点研发指导项目(2017YFC0907400);国家自然科学基金(81770698,81370835);辽宁省重点研发指导计划(2019JH8/10300009)。

摘　　要：目的探讨miR-29a在急性肾损伤(AKI)中的作用。方法 12只雄性CD1小鼠随机分为2组:对照组、叶酸组。检测血清肌酐、尿素氮,PAS染色观察病理变化,TUNEL染色观察细胞凋亡程度,qPCR检测基因IL-6、TNF-α、α-SMA、TGF-β1、miR-29a的表达,Western blot检测α-SMA、TGF-β1蛋白的表达。结果与对照组比,叶酸组血清肌酐、尿素氮升高,PAS染色显示肾小管扩张、刷状缘消失,TUNEL染色提示细胞凋亡增加,IL-6、TNF-α、α-SMA、TGF-β1表达上调,miR-29a表达下调。结论 miR-29可能参与AKI的发生发展。Objective To investigate the role of miR-29 a in acute kidney injury(AKI). Methods Twelve male CD1 mice were randomly divided into: control group and the folic acid group. The serum creatinine and blood urea nitrogen were measured, pathological changes were observed by PAS staining, apoptosis degree was observed with TUNEL staining, the expression of genes IL-6、 TNF-α 、 α-SMA、 TGF-β1 and miR-29 a were measured by qPCR. The expression of α-SMA, TGF-β1 protein were measured by Western blot. Results The contents of serum creatinine and blood urea nitrogen increased, dilation of renal tubules and disappearance of the brush border, apoptosis rate increased, the expression levels of IL-6 and TNF-α and α-SMA and TGF-β1 increased, the expression level of miR-29 a downregulated in folic acid group than in control group. Conclusion miR-29 a might be involved in the development of AKI.

关 键 词：急性肾损伤 miR-29a 炎性因子 凋亡 

分 类 号：R692[医药卫生—泌尿科学]