辣椒苗期抗感黄瓜花叶病毒病比较转录组学分析  

Comparative Transcriptome Analysis of CMV Resistance of Pepper at Seedling Stage

作　　者：雷阳 成妍 乔宁 焦彦生 吴越莉 LEI Yang;CHENG Yan;QIAO Ning;JIAO Yansheng;WU Yueli(College of Horticulture,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China)

机构地区：[1]山西农业大学园艺学院,山西太原030031

出　　处：《中国蔬菜》2021年第7期24-31,共8页China Vegetables

基　　金：山西省青年基金项目(201901D211563);山西省科技成果转化引导专项(201804D131062);山西省农业科学院农业科技创新研究课题/博士后基金项目(YCX2020BH4);山西农业大学学术恢复科研专项(2020xshf25)。

摘　　要：为了研究辣椒在黄瓜花叶病毒侵染下的分子响应机制、发掘抗病相关基因,以感病材料茄门和抗病材料JJ101为对象,对接种黄瓜花叶病毒前后的茄门和JJ101叶片进行高通量转录组测序,并利用生物信息学方法对基因表达和功能进行研究。结果显示,采用RPKM法计算基因表达量,共筛选出JJ101和茄门接种黄瓜花叶病毒后的1158个差异表达基因。其中有643个差异基因在GO(gene ontology)中富集,功能主要涉及酶活性、代谢过程和防御反应等。有287个差异基因归入KEGG通路,在这些通路中,包括植物-病原互作、植物激素信号传导等过程。研究发现,辣椒对黄瓜花叶病毒的免疫是一个极其复杂、多个通路共同调节的过程,蛋白质代谢、防御反应、激素调节等通路均参与其中,为后续深入研究辣椒抗病分子机理奠定了理论基础。In order to explore the molecular response mechanism of pepper under infection of cucumber mosaic virus and resistant genes related to this virus,the paper took susceptible material‘Qiemen’and resistant material‘JJ101’as objectives,and conducted high-throughput transcriptome sequencing on‘Qiemen’and‘JJ101’before and after innoculation of CMV,and carried out studies on gene expression and function by bioinformatics method.The results showed that by using RPKM to calculate gene expression level,1158 differentially expressed genes(DEGs)were screened out after‘Qiemen’and‘JJ101’were inoculated with CMV.Among them,643 DEGs were enriched in Gene Ontology(GO).Their functions were mainly referred to enzyme activity,metabolic process and defense reaction,etc.While 287 DEGs could be included into KEGG pathways,including plant pathogen interaction,plant hormone signal transduction,etc.process.Studies discovered that pepper immunity to CMV was an extreme complicated and multi-pathway coordinated process,jointly participated by protein metabolism,defense response,hormone regulation and the other pathways,laying a theoretical foundation for further research on pepper resistant molecular mechanism.

关 键 词：辣椒 黄瓜花叶病毒 转录组 功能 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]