猕猴桃‘金艳’和‘红阳’果实转录组的比较分析  

Comparative Analysis of’Jinyan’and’Hongyang’Kiwifruit Transcriptomes

作　　者：李贵生[1] LI Guisheng(Department of Biological Resources and Environmental Sciences,Jishou University,the Plant Resources Protection and Utilization Hunan Province College Key Laboratory,Jishou,Hunan 416000,China)

机构地区：[1]吉首大学生物资源与环境科学学院,植物资源保护与利用湖南省高校重点实验室,湖南吉首416000

出　　处：《园艺学报》2021年第6期1183-1196,共14页Acta Horticulturae Sinica

基　　金：吉首大学科研启动基金项目(8811910);湖南省高校科技创新团队支持计划项目(201208Z02)。

摘　　要：转录组比较发现,果肉黄色的‘金艳’猕猴桃成熟果实中优势表达基因的主要功能涉及代谢,而果肉含花青苷呈红色的‘红阳’中的优势表达基因一般与发育相关。猕猴桃果皮和外层果肉部分及内层果肉和果心部分的优势表达基因分别与光合作用和营养储备有关。猕猴桃有相对较多的R2R3类型的MYB基因,几乎所有类型的MYB基因都在果实中表达。MYB-A72位于花青苷合成相关MYB基因分支,其转录本只在‘红阳’中发现,在内层果肉和果心部分的表达水平很高。表达模式聚类分析表明:bHLH-B36和WD40-A126可能参与形成MYB-bHLH-WD40调控复合体,而CHI2、FLS1和CYP98A1可能是受其调控的靶基因。Transcriptome comparison found that preferentially expressed genes(PEGs)were mainly involved in metabolism in the yellow-fleshed ripened‘Jinyan’kiwifruit,while they were generally related to development in red-fleshed’Hongyang’.Meanwhile,PEGs from the peripheral part consisting of the peel and the outer layer of kiwifruits flesh and the remained core flesh part were related to their own hallmark function namely photosynthesis and nutrient reservoir,respectively.Kiwifruit possessed relatively abundant MYB genes of R2R3 type,and nearly all types of MYB genes were expressed in fruits.MYB-A72 transcript could only be found in’Hongyang’,and it possessed extremely high level of expression in the core flesh part,and additionally it was located within the clade including MYB genes for anthocyanin biosynthesis.Expression pattern clustering analysis revealed that bHLH-B36 and WD-A126 might participate in forming the MYB-bHLH-WD40 regulatory complex,and that CHI2,FLS1,and CYP98 A might be the targets under its regulation.

关 键 词：猕猴桃 花青苷 MYB 基因家族 转录组分析 

分 类 号：S633.4[农业科学—蔬菜学]