内蒙古赤峰地区番茄斑萎病毒的鉴定  

Identification of Tomato spotted wilt orthotospovirus in Chifeng,Inner Mongolia

作　　者：杨菲[1] 张爱红[1] 王秀芝[2] 李希望 邸垫平[1] 苗洪芹[1] 张尚卿[3] 孙祥瑞[1] 孙茜[1] YANG Fei;ZHANG Aihong;WANG Xiuzhi;LI Xiwang;DI Dianping;MIAO Hongqin;ZHANG Shangqing;SUN Xiangrui;SUN Qian(Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Baoding,Hebei 071000,China;Chifeng Academy of Agricultural and Animal Sciences,Chifeng,Inner Mongolia 024031,China;Tangshan Academy of Agricultural Sciences,Tangshan,Hebei 063001,China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,河北保定071000 [2]赤峰市农牧科学研究院,内蒙古赤峰024031 [3]唐山市农业科学研究院,河北唐山063001

出　　处：《园艺学报》2021年第6期1208-1216,共9页Acta Horticulturae Sinica

基　　金：河北省现代农业产业技术体系蔬菜产业创新团队项目(HBCT2018030207);河北省农林科学院基本科研业务费项目(208120102)。

摘　　要：利用番茄斑萎病毒(tomato spotted wilt orthotospovirus,TSWV)通用引物及自行设计合成引物对采集自内蒙古自治区赤峰市部分地区的番茄病毒病样品进行了RT-PCR检测及序列测定;进一步利用特异性引物对包含N基因在内核苷酸序列进行扩增、克隆和测序,并对包含N基因的914 nt序列进行系统进化分析。结果表明,在采集的16个表现TSWV症状和2个未表现典型症状的果实样品中均检测到TSWV;TSWV内蒙古番茄分离物与中国云南、江苏等地的分离物亲缘关系最近。用表现TSWV症状的番茄果实汁液摩擦接种辣椒叶片,出现叶片褪绿、植株矮小等症状,并经RT-PCR分析检测到TSWV。本研究证实在内蒙古地区有TSWV在番茄上发生危害。In this study,both tomato spotted wilt orthotospovirus(TSWV)universal primers and TSWV synthetic primers were used to detect the virus disease samples collected from parts of Chifeng,Inner Mongolia China,and the amplified sequences were analyzed.Then,the nucleotide sequence containing N gene amplified by using specific primers was sequenced for phylogenetic analysis.The results showed that TSWV was detected in all 18 tomato fruit samples.The phylogenetic analysis of sequences including 914 nt fragment containing N gene showed that TSWV tomato isolate from Inner Mongolia was more closely to isolates from Yunnan and Jiangsu.After sap inoculation,pepper showed the symptoms of chlorosis in leaf and dwarfing.TSWV was detected by RT-PCR on the symptomatic leaves of pepper.This is the first time to prove that TSWV is harmful to tomato by molecular methods in Inner Mongolia.

关 键 词：番茄 番茄斑萎病毒 RT-PCR检测 内蒙古 

分 类 号：S641.2[农业科学—蔬菜学]