岗松总黄酮对宫颈癌SiHa细胞迁移、侵袭及凋亡的影响  

Effects of total flavonoids from Baeckea frutescens on the migration,invasion and apoptosis of cervical cancer SiHa cells

作　　者：马秀珍 卢艳[2] 赵冰冰[2] 邱宏聪 徐勋 韦敏[1] Ma Xiuzhen;Lu Yan;Zhao Bingbing;Qiu Hongcong;Xu Xun;Wei Min(Graduate School of Guangxi Medical University,Nanning 530021,China;Department of Gynecological Oncology,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,China;Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning 530022,China)

机构地区：[1]广西医科大学研究生院,南宁530021 [2]广西医科大学附属肿瘤医院妇瘤科,南宁530021 [3]广西壮族自治区中医药研究院,南宁530022

出　　处：《国际肿瘤学杂志》2021年第4期206-211,共6页Journal of International Oncology

基　　金：广西中药质量标准研究重点实验室(广西壮族自治区中医药研究院)开放基金(桂中重开201303);中国民族医药学会科研项目(2019KYXM-M246-46)。

摘　　要：目的研究岗松总黄酮对宫颈癌SiHa细胞增殖、迁移、侵袭、凋亡及细胞形态改变的影响。方法用不同浓度的岗松总黄酮对宫颈癌SiHa细胞进行处理,采用CCK8法检测SiHa细胞体外增殖情况和半抑制浓度(IC50)。设置不做药物处理的对照组与药物浓度为IC50值处理的实验组。Transwell小室迁移、侵袭实验分别检测实验组与对照组细胞体外迁移、侵袭能力的变化;激光共聚焦显微镜下观察实验组与对照组细胞体外凋亡形态变化;流式细胞术检测实验组与对照组细胞凋亡率。结果岗松总黄酮可抑制宫颈癌SiHa细胞增殖,具有浓度依赖性,作用48 h后其IC50值为110.8 mg/L。迁移实验中对照组穿膜细胞数为(644.00±10.54)个,实验组穿膜细胞数为(266.00±5.57)个,差异有统计学意义(t=54.942,P<0.001)。侵袭实验中对照组穿膜细胞数为(298.00±14.36)个,实验组穿膜细胞数为(85.00±8.62)个,差异有统计意义(t=38.247,P<0.001)。激光共聚焦显微镜观察发现,实验组可见细胞膜皱缩失去原有的形态,细胞核呈现大小不等、不规则形态的碎片,核边集等典型的凋亡形态;对照组未见凋亡形态细胞。流式细胞术检测结果显示,对照组凋亡率为(2.95±1.36)%,实验组凋亡率为(27.54±1.94)%,差异有统计学意义(t=-17.949,P<0.001)。结论岗松总黄酮对体外培养的宫颈癌SiHa细胞增殖、迁移、侵袭具有明显的抑制作用,并促进其凋亡。Objective To study the effects of total flavonoids from Baeckea frutescens on the proliferation,migration,invasion,apoptosis and cell morphology changes of cervical cancer SiHa cells.Methods Cervical cancer SiHa cells were treated with different concentrations of total flavonoids from Baeckea frutescens.CCK8 method was used to detect the proliferation and 50%inhibiting concentration(IC50)of SiHa cells in vitro.The control group without drug treatment and the experimental group with drug concentration IC50 were set.Transwell migration and invasion experiments were used to detect the changes of cell migration and invasion ability in vitro in the experimental group and the control group.Laser scanning confocal microscope was adopted for observing the morphological changes of apoptosis in the experimental group and the control group.Flow cytometry was used to detect the apoptosis rates of the experimental group and the control group.Results Total flavonoids from Baeckea frutescens inhibited the proliferation of cervical cancer SiHa cells in a concentration-dependent manner.After 48 hours of action,the IC50 value was 110.8 mg/L.In the migration experiment,the number of transmembrane cells in the control group was 644.00±10.54 and the number of transmembrane cells in the experimental group was 266.00±5.57,with a statistically significant difference(t=54.942,P<0.001).In the invasion experiment,the number of transmembrane cells in the control group was 298.00±14.36,and the number of transmembrane cells in the experimental group was 85.00±8.62,with a statistically significant difference(t=38.247,P<0.001).Laser scanning confocal microscope observation showed that in the experimental group,the cell membrane crumpled and lost its original morphology,and the nucleus showed typical apoptotic morphologies such as fragments of different sizes and irregular shapes,and nuclear edge aggregation;but no apoptotic cells were observed in the control group.Flow cytometry showed that the apoptosis rate in the control group was(2.95±

关 键 词：宫颈肿瘤 细胞增殖 肿瘤浸润 细胞凋亡 岗松总黄酮 

分 类 号：R285[医药卫生—中药学]