A single-cell atlas of mouse olfactory bulb chromatin accessibility  

作　　者：Yin Chen Xiangning Ding Shiyou Wang Peiwen Ding Zaoxu Xu Jiankang Li Mingyue Wang Rong Xiang Xiaoling Wang Haoyu Wang Qikai Feng Jiaying Qiu Feiyue Wang Zhen Fluang Xingliang Zhang Gen Tang Shengping Tang 

机构地区：[1]BGI Education Center,University of Chinese Academy of Sciences,Shenzhen 518083,China [2]BGI-Shenzhen,Shenzhen 518083,China [3]School of Biological Science&Medical Engineering,Southeast University,Nanjing 210096,China [4]Southern Center for Biomedical Research and Fujian Key Laboratory of Developmental and Neural Biology,College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China [5]Shenzhen Children’s Hospital,Shenzhen 518083,China [6]Department of Pediatrics,the Affiliated Hospital of Guangdong Medical University,Zhanjiang 524000,China [7]Zunyi Medical University,Zunyi,Guizhou 563099,China [8]China Medical University,Shenyang,Liaoning 110122,China

出　　处：《Journal of Genetics and Genomics》2021年第2期147-162,共16页遗传学报（英文版）

基　　金：supported by Shenzhen Sanming Engineering Project(SZSM202011012);Shenzhen Innovation Science and Technology Committee(JCYJ20180228175358223);National Natural Science Foundation of China(31670742)。

摘　　要：Olfaction,the sense of smell,is a fundamental trait crucial to many species.The olfactory bulb(OB)plays pivotal roles in processing and transmitting odor information from the environment to the brain.The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing.However,the epigenetic landscape of the m OB remains mostly unexplored.Herein,we apply single-cell assay for transposaseaccessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the m OB.Based on single-cell epigenetic signatures,m OB cells are classified into 21 clusters corresponding to 11 cell types.We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred.In addition,the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage.Our study provides a valuable epigenetic data set for the m OB at single-cell resolution,and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.

关 键 词：scATAC-seq Mouse olfactory bulb Chromatin accessibility Transcription factor Endogenous retroviral elements Co-embedding analysis 

分 类 号：Q434[生物学—生理学]