基于Nrf2/HMOX-1铁死亡信号通路探讨番泻叶苷A治疗糖尿病肾病的机制  

Mechanism Exploration of Sennoside A in Treating DN Based on Nrf2/HMOX-1 Ferroptosis Signaling Pathway

作　　者：丁阳 王利 DING Yang;WANG Li(Shanghai Liqun Hospital of Putuo District,Shanghai 200333,China;Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海市普陀区利群医院,上海200333 [2]上海市中医医院,上海200071

出　　处：《中医药信息》2021年第7期36-39,共4页Information on Traditional Chinese Medicine

基　　金：中医临床重点专科项目(PTZYLCZDZK-2017007);上海市第三批中医临床优势专科建设项目(ZYSNXD-YL-YSZK024)。

摘　　要：目的:探究番泻叶苷A对糖尿病肾病铁死亡的作用机制。方法:6周龄雄性C57BL/6J小鼠分为正常对照组和造模组。造模成功小鼠分为番泻叶苷A(SA)组和模型组,各10只。Western Blot法检测Nrf2、HMOX-1、GPX4的蛋白变化情况;GSH和MDA试剂盒检测氧化应激水平;DAB染色法检测PTGS2阳性细胞表达。结果:模型组Nrf2、HMOX-1蛋白表达量明显升高,GPX4表达显著降低;PTGS2阳性细胞表达明显增加;MDA水平显著增加、GSH活性明显降低。番泻叶苷A组明显改善氧化应激反应,下调Nrf2、HMOX-1、PTGS2的表达,增加GPX4的表达。结论:糖尿病肾病存在铁死亡,番泻叶苷A可以抑制铁死亡水平,其机制可能与抑制Nrf2/HMOX-1信号通路有关。Objective:To explore the mechanism of sennoside A on ferroptosis in the treatment of diabetic nephropathy(DN).Methods:Six weeks old male C57 BL/6 J mice were divided into the normal control group and the model group.The successful model mice were divided into Sennoside A group and the model group,with 10 mice in each group.Western blot method was used to detect the protein changes of Nrf2,HMOX-1 and GPX4;GSH and MDA kits were used to detect the level of oxidative stress;DAB staining method was used to detect the expression of PTGS2 positive cells.Results:In the model group,the protein expressions of Nrf2 and HMOX-1 were significantly increased,the expression of GPX4 was significantly decreased,the expression of PTGS2 positive cells was significantly increased,the level of MDA was significantly increased,and the activity of GSH was significantly decreased.Sennoside A significantly improved the oxidative stress response,down-regulated the expressions of Nrf2,HMOX-1 and PTGS2,and increased the expression of GPX4.Conclusion:Sennoside A can inhibit the level of ferroptosis in the treatment of DN,which mechanism may be related to the inhibition of Nrf2/HMOX-1 signaling pathway.

关 键 词：糖尿病肾病 铁死亡 番泻叶苷A Nrf2/HMOX-1 

分 类 号：R285.5[医药卫生—中药学] R259[医药卫生—中医学]