氨基酸影响尖孢镰孢菌古巴专化型厚垣孢子的形成  

Amino acid involved in chlamydospore formation of Fusarium oxysporum f. sp. cubense

作　　者：丁兆建 漆艳香[2] 曾凡云[2] 朱为菊[1] 许天委 彭军 谢艺贤[2] 张欣[2] DING Zhao-Jian;QI Yan-Xiang;ZENG Fan-Yun;ZHU Wei-Ju;XU Tian-Wei;PENG Jun;XIE Yi-Xian;ZHANG Xin(Department of Biological Science,Qiongtai Normal University,Haikou,Hainan 571127,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Tropical Biodiversity and Bioresource Utilization Laboratory,Qiongtai Normal University,Haikou,Hainan 571127,China)

机构地区：[1]琼台师范学院生物科学系,海南海口571127 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101 [3]琼台师范学院热带生物多样性与资源利用实验室,海南海口571127

出　　处：《菌物学报》2021年第6期1413-1426,共14页Mycosystema

基　　金：国家自然科学基金(31801690);2019年海南省基础与应用基础研究计划高层次人才项目(2019RC249);现代农业产业技术体系专项(CARS-31-07);琼台师范学院校级项目(qtyb201910)。

摘　　要：香蕉枯萎病是由尖孢镰孢菌古巴专化型Fusarium oxysporum f. sp. cubense(Foc)侵染引起的一种土传真菌病害,已严重威胁香蕉产业的健康发展。该病菌产生的厚垣孢子可在土壤中存活多年,是香蕉枯萎病的初侵染源。本研究通过氨基酸添加试验,证明添加甘氨酸可抑制厚垣孢子的形成;通过对该病菌厚垣孢子形成前期、初期、中期和后期的转录组分析,发现氨基酸合成通路中有93个基因的表达水平在厚垣孢子形成过程中发生了显著变化;In silico分析表明其中10个基因参与调控真菌的氨基酸合成,11个基因参与调控真菌种的生长发育和产孢,19个基因参与调控真菌种的致病性和毒素产生。由此推测,氨基酸合成通路不仅与尖孢镰孢菌古巴专化型厚垣孢子的形成相关,其有可能参与调控该病菌的致病性。Fusarium wilt of banana is a devastative soil-borne fungal disease, caused by Fusarium oxysporum f. sp. cubense(Foc) and it is a serious threat to health production of banana. Chlamydospores abundantly produced by Foc could survive for many years in the infected-soil as primary infection sources of the disease. The experimental results indicate that chlamydospore formation of Foc is evidently inhibited by supplement of glycine in the induction system. Transcriptome analyses of mycelial growth, and chlamydospore formation in initial and afterwards developing stages revealed that expression levels of 93 genes varyied in amino acid biosynthesis pathway. Among them, 10 genes were involved in the regulation of fungal amino acid biosynthesis, 11 genes involved in the regulation of fungal growth and conidial formation, and the other 19 genes in the regulation of fungal virulence and toxin production. These results confirm that glycine is involved in chlamydospore formation and virulence of Foc.

关 键 词：香蕉枯萎病 甘氨酸 形态发育 差异表达基因 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]