猴头菌和金针菇漆酶对不同染料的降解  

Degradation of different dyes by laccases from Hericium erinaceus and Flammulina filiformis

作　　者：徐圣东 周金洋 王丽[1] 朱孟娟 XU Sheng-Dong;ZHOU Jin-Yang;WANG Li;ZHU Meng-Juan(College of Plant Protection,Shandong Agricultural University,Tai’an,Shandong 271018,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《菌物学报》2021年第6期1525-1537,共13页Mycosystema

基　　金：国家自然科学基金(31501813)。

摘　　要：利用漆酶(laccase)处理染料废水是近年来研究的热点。本研究以猴头菌Hericium erinaceus和金针菇Flammulina filiformis的发酵液为试验材料,通过硫酸铵沉淀、离子交换层析和超滤等方法,对发酵液中的漆酶进行了初步的分离纯化,然后分别研究了两种初提纯漆酶及其与小分子介体组成的漆酶介体系统(laccase-mediator system,LMS)对12种常用染料的降解效果。结果表明,猴头菌的初提纯漆酶对甲基红、铬黑T、孔雀绿和活性蓝R都具有很好的降解效果,反应24h后,降解率分别为79.6%、66.8%、80.3%和64.6%,且表现出时间依赖性,而对其他染料的降解效果不明显。当反应体系中加入介体2,2′-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐[2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphate),ABTS]后,可增加猴头菌漆酶对考马斯亮蓝、结晶紫和孔雀绿的降解率,分别提高到33.7%、45.6%和94.2%。金针菇初提纯漆酶对孔雀绿、活性艳橙K-7R和活性红KD-8B都具有一定的降解效果,降解率分别为37.8%、39.9%和49%,也表现出了明显的时间依赖性。当反应体系中也加入ABTS后,金针菇漆酶对活性红KM-8B的降解率明显增加,由9.7%增加到58.2%。以上研究为猴头菌和金针菇漆酶在染料废水处理领域的应用提供了科学依据。Treatment of dye wastewater by laccase becomes a research hotspot in recent years. In this study, laccase from the fermentation broth of Hericium erinaceus and Flammulina filiformis was preliminarily separated and purified successively by ammonium sulfate precipitation, ion exchange chromatography and ultrafiltration. The degradation effects of the primary purified laccases and the laccase-mediator system(LMS) on 12 commonly used dyes were studied respectively. The results showed that the primary purified laccase from H. erinaceus had good degradation effect on methyl red, chrome black T, malachite green and reactive blue R, and the degradation rates were 79.6%, 66.8%, 80.3% and 64.6% in 24 h, respectively, showing time-dependent, but the degradation effect on other dyes was not obvious. When 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphate)(ABTS) was added to the reaction system, the degradation rates of coomassie brilliant blue, crystal violet and malachite green by H. erinaceus laccase increased by 33.7%, 45.6% and 94.2%, respectively. The F. filiformis laccase had degradation effects to a certain extent on peacock green, reactive brilliant orange K-7 R and reactive red KD-8 B, and the degradation rate was 37.8%, 39.9% and 49%, respectively, also showing time dependence. When ABTS was added to the reaction system, the degradation rate of reactive red KM-8 B by F. filiformis laccase increased significantly from 9.7% to 58.2%. The studies provide scientific basis for the application of H. erinaceus laccase and F. filiformis laccase in the field of dye wastewater treatment.

关 键 词：白腐真菌 漆酶介体系统 染料降解 

分 类 号：X703[环境科学与工程—环境工程]