被子植物小热激蛋白家族的比较基因组学分析  

Comparative Genomics of the Small Heat Shock Protein Family in Angiosperms

作　　者：范凯 叶方婷 毛志君 潘鑫峰 李兆伟 林文雄 Kai Fan;Fangting Ye;Zhijun Mao;Xinfeng Pan;Zhaowei Li;Wenxiong Lin(Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]福建农林大学农学院,作物遗传育种与综合利用教育部重点实验室,福州350002

出　　处：《植物学报》2021年第3期245-261,共17页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31701470);中国博士后基金(No.2017M610388,No.2018T110637);福建农林大学杰出青年科研人才计划(No.xjq201917);福建农林大学科技创新专项基金(No.CXZX2020007A)。

摘　　要：小热激蛋白(sHSP)是一类重要的响应外界环境变化以及调控植物生长发育的蛋白家族。基于在睡莲(Nymphaea colorata)、水稻(Oryza sativa)、拟南芥(Arabidopsis thaliana)和葡萄(Vitis vinifera)中分别鉴定到的33个NcsHSPs、24个OssHSPs、17个AtsHSPs和47个VvsHSPs,表明sHSP家族可分为12个亚家族,不同亚家族包含不同的sHSP成员数目、保守基序、基因结构以及复制基因数目。在4种模式被子植物的sHSP成员中共鉴定到12个基因复制事件,片段复制事件和串联复制事件均与sHSP成员的扩增有关,且片段复制事件发生的时间早于串联复制事件。在所有sHSP成员中,拟南芥和葡萄的sHSP成员的同源性最高,其次为睡莲和葡萄的sHSP成员。sHSP家族在被子植物中可能向更短的氨基酸长度、更小的分子量、更简单的基因结构以及更集中的染色体分布进化。此外,在睡莲、水稻、拟南芥和葡萄中鉴定了一些可能与调控植物生长发育相关的候选基因。研究结果为4种模式被子植物sHSP家族的比较基因组学研究奠定了重要基础,并为其它被子植物sHSP家族的研究提供重要参考。Small heat shock protein(s HSP) can respond to environmental stresses and regulate plant growth and development. In this study, 33 NcsHSPs, 24 OssHSPs, 17 AtsHSPs and 47 VvsHSPs were found in waterlily, rice, Arabidopsis, and grape, respectively. The sHSP family can be divided into 12 subfamilies, and different subfamily had different number of sHSP members, conserved motifs, gene structures and number of duplicated genes. Then, 12 gene duplication events in tle s HSP fanily were identified from 4 model angiosperms of s HSP family. The segmental and tandem duplication events were related to the gene expansion in the sHSP family, and the segmental duplication events occurred earlier than the tandem duplication events. Moreover, sHSP members in Arabidopsis and grape had the highest orthologous similarity, followed by sHSP members in waterlily and grape. Furthermore, sHSP family in angiosperms evolved towards sHSP members with smaller length of amino acid, lower molecular weight, simpler gene structure, and concentrated chromosomal localization. Besides, some s HSP members may be related to plant growth and development in waterlily, rice, Arabidopsis, and grape. Above all, these results could lay vital foundations on comparative genomics of the sHSP family in four model angiosperms and advance the sHSP family in other angiosperms.

关 键 词：sHSP家族 比较基因组学 扩增 基因表达 

分 类 号：Q943.2[生物学—植物学]