外源海藻糖增强高表达转玉米C4型PEPC水稻耐旱性的机制  

Mechanism on Drought Tolerance Enhanced by Exogenous Trehalose in C-PEPC Rice

作　　者：李佳馨 李霞 谢寅峰[1] Jiaxin Li;Xia Li;Yinfeng Xie(College of Biology and Environment,Nanjing Forestry University,Nanjing 210037,China;Nanjing Branch of China National Center for Rice Improvement/Jiangsu High Quality Rice Engineering Technology Research Center/Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Collaborative Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province,Agricultural College,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]南京林业大学生物与环境学院,南京210037 [2]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京210014 [3]扬州大学农学院,江苏省粮食作物现代产业技术协同创新中心,扬州225009

出　　处：《植物学报》2021年第3期296-314,共19页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31571585);国家重点研发计划(No.2016YFD0300501-03);江苏省重点研发计划(现代农业)(No.BE2019377)。

摘　　要：为揭示海藻糖(Tre)调控转玉米(Zea mays)C4型PEPC基因水稻(Oryza sativa)(PC)的耐旱性机制,以PC及其野生型Kitaake(WT)为材料,通过水培试验,研究了Tre和12%(m/v)聚乙二醇(PEG)单独或联合处理对水稻生理生化特性的影响。结果表明,Tre处理可促进PC和WT水稻幼苗生长,缓解干旱逆境导致的植株生长抑制,但对PC的效应更显著。与DS处理相比,Tre+DS联合处理可维持功能叶较高的相对含水量、光化学效率和抗氧化酶活性。在DS处理下,与WT相比,外施Tre可使PC的内源Tre和蔗糖含量显著增加,而葡萄糖含量显著降低,Tre代谢和SnRK1s相关基因表达量增加;施用Tre也显著促进了ABA合成、信号转导与干旱响应基因的表达,和维持较稳定的光合能力,从而使PC表现更强的耐旱性。In order to reveal the mechanism of trehalose(Tre)involved in the drought tolerance of transgenic rice(Oryza sativa)(PC)expressing the C4-PEPC gene of maize(Zea mays),we analyzed the physiological and biochemistry characteristics of PC and wild-type rice(WT)treated hydroponically with Tre and 12%(m/v)PEG alone or in combination.The results showed that Tre treatment promoted the growth of PC and WT seedlings,and alleviated the inhibition of plant growth caused by drought stress(DS),with the effect being more significant for PC.Compared with DS treatment,Tre+DS treatment maintained functional leaves a higher relative water content,photochemical efficiency and antioxidant enzyme activity.Under DS,compared with WT,PC showed significant increase in the content of Tre and sucrose and decrease in the content of glucose,and up-regulated expression of genes associated with Tre metabolism and SnRK1 s after Tre application.Tre application also improved PC the ABA synthesis,and expression of genes related to signal transduction and drought response,and maintained PC a relatively stable photosynthetic capacity,thus possibly conferring stronger drought tolerance of PC.

关 键 词：转C4-PEPC基因水稻 干旱胁迫 磷酸烯醇式丙酮酸羧化酶 海藻糖 水稻 

分 类 号：S511[农业科学—作物学]