基于MRM-IDA-EPI技术筛查猪尿中4种β-受体激素残留  

Simultaneous determination of 4β-receptor hormone residues in swine urine by MRM-IDA-EPI method

作　　者：汤逸飞 屠雨晨 陈欣慰 黄芳 张国平 Tang Yifei;Tu Yuchen;Chen Xinwei;Huang Fang;Zhang Guoping(Jiaxing Center for Inspection and Testing of Agriculture,Jiaxing 314050,China)

机构地区：[1]嘉兴市农业综合检验检测中心,浙江嘉兴3140501

出　　处：《农业科学研究》2021年第2期18-21,共4页Journal of Agricultural Sciences

基　　金：嘉兴市农业综合检验检测中心运行经费(072013001);嘉兴市公益性研究计划项目(2021AY10041)。

摘　　要：建立了猪尿中克伦特罗、莱克多巴胺、沙丁胺醇和特布他林4种β-受体激素残留同时测定的快速前处理-超高效液相(QuEChERS-UPLC)/多反应监测-信息依赖-增强型质谱扫描(MRM-IDA-EPI)的一种方法。10.0 mL猪尿用乙腈溶液提取,乙二胺-N-丙基硅烷、石墨化炭黑、硫酸镁和氯化钠净化后直接进样分析,采用+MRM-IDA-EPI模式,外标法定量。结果表明:4种β受体激素在0.5~50μg/L的范围内线性关系良好,回归方程相关系数(r)在0.985~0.994之间,样品在添加质量浓度的平均回收率(p%)为62.11%~112.80%,相对标准偏差(RSD%)为0.63%~14.38%,其定量方法准确度、精密度均可以相对满足要求。同时,该方法可通过同步EPI结构解析,复核4种β-受体激素[M+H]+等特征碎片离子的检出,可为猪尿中β-受体激素残留的监控和风险评估等提供一种可靠且高效的分析手段。A QuEChERS sample pretreatment method coupled with QuEChERS-UPLC/MRM-IDA-EPI was established to determine 4β-receptor hormone residues in swine urine.10.0 mL of swine urine was extracted by homogenization with aceonitrile and the extract was cleaned up with PSA,GCB,MgSO_(4) and NaCl to remove the interferences from extractives.The target compounds were detected by electron impact ionization in+MRM mode with external standard method.The result showed that the method appeared to be a good linear relationship in the concentration ranges of 0.5~50μg/L,with correlation coefficients(r)between 0.985 and 0.994.The average recoveries(p)of this method were in the range of 62.11%~112.80% with relative standard deviations(RSD)between 0.63% and 14.38%.Moreover,the method could be detected[M+H]+and other characteristic fragment ions through EPI structure analysis on 4β-receptor hormone residues in swine urine,which could be analyzed for the monitoring and risk assessment fast,efficiently and reliably.

关 键 词：QuEChERS-UPLC MRM-IDA-EPI 猪尿 β-受体激素 

分 类 号：S859.8[农业科学—临床兽医学]