滇青冈(Cyclobalanopsis glaucoides)内生菌及根际土壤微生物的初步研究  

Preliminary Study on the Endophytes and Rhizosphere Soil Microbes of Cyclobalanopsis glaucoides

作　　者：杨海艳[1] 李艳双 李蕊君 张燕 罗中泽 李天星[1] Haiyan Yang;Yanshuang Li;Riujun Li;Yan Zhang;Zhongze Luo;Tianxing Li(School of Resources,Environment&Chemistry,Chuxiong Normal University,Chuxiong,Yunnan Province 675000;College of Language and Culture,Chuxiong Normal University,Chuxiong,Yunnan Province 675000;Institute of Cash Crops,Chuxiong Academy of Agricultural Sciences,Chuxiong 675000,China)

机构地区：[1]楚雄师范学院资源环境与化学学院,云南楚雄675000 [2]楚雄师范学院语言文化学院,云南楚雄675000 [3]楚雄州农业科学院经济作物研究所,云南楚雄675000

出　　处：《楚雄师范学院学报》2021年第3期67-79,共13页Journal of Chuxiong Normal University

基　　金：National natural science foundation of China(No.31360102).

摘　　要：实验对滇青冈根、茎、叶的内生菌及根际土壤微生物进行了初步研究,结果表明:从滇青冈根、茎、叶及根际土壤中分离出细菌61株,真菌24株,共85株。根中分离出了29株,包括25株细菌,4株真菌;茎中12株,包括4株细菌,8株真菌;叶中7株,包括3株细菌,4株真菌,即根(34%)>茎(14%)>叶(8%),其余37株都是从土壤中分离出来的。细菌在滇青冈体内分布情况为根(56%)>茎(8%)>叶(6%),真菌则是茎中较多。从根、茎、叶中分离出的细菌多为球菌,根际土壤中分离出的则是杆菌较多,二者分离出的菌株都是G+占优势。分离出的真菌包括2株曲霉属真菌、2株枝孢属、1株凸脐蠕孢属、1株葡萄孢属、1株炭疽菌属、4株青霉属、2株团黑孢霉属,其他的为无孢类群,其中青霉属真菌略占优势。实验结果为滇青冈的化感作用研究提供部分数据支持。The results showed that 61 strains of bacteria and 24 strains of fungi were isolated from the root,stem,leaf and rhizosphere soil of Cyclobalanopsis glaucoides 29 strains were isolated from roots,including 25 strains of bacteria and 4 strains of fungi;12 strains in stems,including 4 strains of bacteria and 8 strains of fungi;7 strains in leaves,including 3 strains of bacteria and 4 strains of fungi,namely root(34%)>stem(14%)>leaf(8%),and the remaining 37 strains were isolated from soil.The distribution of bacteria in C.glaucoides was as follows:root(56%)>stem(8%)>leaf(6%).Fungi were more in stem.Most of the bacteria isolated from roots,stems and leaves were cocci,while the bacteria isolated from rhizosphere soil were more than those from rhizosphere soil.The isolated fungi included 2 strains of Aspergillus,2 strains of Cladosporium,1 strain of Verrucaria,1 strain of botryosporium,1 strain of Colletotrichum,4 strains of Penicillium and 2 strains of Colletotrichum.The others were non spore groups,among which Penicillium was slightly dominant.The results of this study can provide support for the experiment of C.glaucoides.

关 键 词：滇青冈 内生菌 根际土壤微生物 

分 类 号：S154.3[农业科学—土壤学]