温度对烟草黑胫病菌致病力及代谢表型的影响  

Effects of temperature on pathogenicity and metabolic phenotype of tobacco black shank pathogen Phytophthora nicotianae

作　　者：刘畅 向立刚 汪汉成 曾陨涛 何永福 余知和[1] Liu Chang;Xiang Ligang;Wang Hancheng;Zeng Yuntao;He Yongfu;Yu Zhihe(College of Life Sciences,Yangtze University,Jingzhou 434025,Hubei Province,China;Guizhou Academy of Tobacco Sciences,Guiyang 550081,Guizhou Province,China;Guizhou Provincial Tobacco Monopoly Bureau,Guiyang 550003,Guizhou Province,China;Guizhou Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou Province,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]贵州省烟草科学研究院,贵阳550081 [3]贵州省烟草专卖局,贵阳550003 [4]贵州农业科学院植物保护研究所,贵阳550006

出　　处：《植物保护学报》2021年第3期669-678,共10页Journal of Plant Protection

基　　金：国家自然科学基金(31960550);中国烟草总公司贵州省公司科技项目(201914);中国烟草总公司科技项目(110202001035(LS-04))。

摘　　要：为揭示温度对烟草黑胫病菌Phytophthora nicotianae致病力及代谢表型的影响,采用菌丝生长速率法和离体叶片法分别测定不同温度下烟草黑胫病菌的生长速率和致病力,同时采用Biolog代谢表型技术测定其在20、27、30和35℃下的渗透压和pH代谢表型。结果表明,烟草黑胫病菌在21~35℃范围内生长良好且具致病力,30℃时生长速率最快,为17.72 mm/d,30℃和35℃时致病力较强,叶片接种烟草黑胫病菌后24 h内便出现病斑,随温度升高病害的潜伏期缩短。温度影响烟草黑胫病菌的渗透压和pH适应性;27℃和30℃时烟草黑胫病菌的渗透压适应范围最广,其次为21℃,35℃时烟草黑胫病菌渗透压适应范围最窄;在20、27和30℃时,烟草黑胫病菌在pH 5.0~10.0范围内可正常代谢,在35℃时pH代谢范围为5.5~10.0。Mycelial growth of tobacco black shank pathogen Phytophthora nicotianae on LBA plates and its pathogenicity to detached tobacco leaves were first determined under different temperatures.Then, metabolic phenotypes of P. nicotianae under different osmotic pressures and pHs were analyzed using biolog phenotype microarray(PM). The results showed that P. nicotianae grew well and was pathogenic under temperatures ranging from 21℃ to 35℃, with the highest growth rate equal 17.72 mm/d at 30℃;its pathogenicity were the greater at 30℃ and 35℃ than at other temperatures, and the lesions appeared within 24 hours after inoculation;and the latent period shortened with the increase of incubation temperature. Temperature also affected the metabolic phenotypes of the pathogen at different osmotic pressures and pHs. At 27℃ and 30℃, the osmotic adaptation range of P. nicotianae was the widest, followed by 21℃. The osmotic adaptation range of the pathogen was the narrowest at 35℃. At 20,27 and 30℃, P. nicotianae exhibited active metabolism at p H values ranging from 5.0 to 10.0;while at35℃, active metabolism was observed at pH values ranging from 5.5 to 10.0.

关 键 词：烟草疫霉 温度 生长速率 离体致病力 代谢图谱 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]