独角莲抗肿瘤活性成分初步研究  

Effective components of Typhonium giganteum Engl.with anti-tumor activity

作　　者：宁停波 李涛 姜宇珺 梁红宝 姚景春 张贵民 NING Ting-bo;LI Tao;JIANG Yu-jun;LIANG Hong-bao;YAO Jing-chun;ZHANG Gui-min(Shandong New Times Pharmaceutical Co.,Ltd.,Linyi Shandong 273400;Lunan Pharmaceutical Group Co.,Ltd.State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Linyi Shandong 276006)

机构地区：[1]山东新时代药业有限公司,山东临沂273400 [2]鲁南制药集团股份有限公司中药制药共性技术国家重点实验室,山东临沂276006

出　　处：《中南药学》2021年第6期1067-1070,共4页Central South Pharmacy

摘　　要：目的对独角莲的抗肿瘤活性成分进行初步研究。方法通过抑瘤活性检测追踪活性部位,采用水提醇沉法以及离子交换树脂法对独角莲水提物进行HPLC分离纯化。结果活性追踪法研究结果显示,离子交换树脂分离纯化组分Fr.2和Fr.4对小鼠S180实体瘤具有显著的抑瘤活性;组分Fr.4经HPLC纯化得单体化合物1,通过MS、NMR以及与对照品比对确定其为L-精氨酸。用同等剂量的L-精氨酸对照品作对照,发现抑瘤活性与化合物1基本一致。结论初步证明独角莲抗肿瘤活性的有效成分之一是L-精氨酸。Objective To determine the effective components from the water extracts of Typhonium giganteum Engl.with anti-tumor activity.Methods The active parts were purified by comprehensive techniques including alcohol sedimentation,ion-exchange resin,HPLC and bioassay-guided method(S180 tumor-bearing mice model).Results Purified by anion exchange resin fractions Fr.2 and Fr.4,exhibited obvious anti-tumor activity on sarcoma-180 ascite tumor-bearing mice.Fraction Fr.4 was purified by HPLC to obtain compound 1,which was identified as L-arginine by comparison with MS and NMR spectroscopic data with those reported in the literature,and with the reference standard.With the same dose of L-arginine as control,its anti-tumor activity was consistent with that of compoud 1.Conclusion L-arginine is one of the effective anti-cancer components of Typhonium giganteum Engl..

关 键 词：独角莲 抗肿瘤活性 离子交换树脂 活性追踪 L-精氨酸 

分 类 号：R284.1[医药卫生—中药学]