异叶青兰总黄酮的含量测定及体外抗氧化活性研究  

Content determination of total flavonoids and its in vitro antioxidant activity of Dracocephalum heterophyllum Benth

作　　者：马雪洁 程路峰[1] 杨淑梅[1] 何雯 MA Xue-jie;CHENG Lu-feng;YANG Shu-mei;HE Wen(College of Pharmacy,Xinjiang Medical University,Urumqi 830011;Chengdu Fifth People’s Hospital,Chengdu 611130)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]成都市第五人民医院,成都611130

出　　处：《中南药学》2021年第6期1130-1133,共4页Central South Pharmacy

基　　金：国家自然科学基金(No.82004066,No.81560689);新疆维吾尔自治区自然科学基金(No.2016D01C225)。

摘　　要：目的测定异叶青兰总黄酮的含量并对其进行体外抗氧化活性研究。方法采用70%乙醇加热回流提取异叶青兰总黄酮,采用硝酸铝比色法测定其总黄酮含量。以维生素C为阳性对照,检测异叶青兰总黄酮清除DPPH、ABTS自由基能力及总抗氧化能力,评价其体外抗氧化活性。结果异叶青兰总黄酮平均含量为48.6%,得率为3.6%。其对DPPH和ABTS自由基的清除率可高达82.94%和98.48%,IC50值分别为0.063 mg·mL^(-1)和0.079 mg·mL^(-1),总抗氧化能力达5.81μmol·mL^(-1)。结论该方法简单,重复性好,可用于异叶青兰总黄酮的含量测定。异叶青兰总黄酮具有良好的抗氧化活性。Objective To determine the content and antioxidant activity in vitro of total flavonoids of Dracocephalum heterophyllum Benth.Methods Ethanol(70%)was used to reflux the total flavonoids in Dracocephalum heterophyllum Benth.Through the aluminum nitrate colorimetric method,the total flavonoid content was determined.Vitamin C was used as the positive control,and the anti-oxidant activity in vitro was evaluated by the scavenging ability of the total flavonoids of Dracocephalum heterophyllum Benth on DPPH and ABTS free radicals and the total antioxidant capacity.Results The average content of total flavonoids in Dracocephalum heterophyllum Benth was 48.6%,and the yield was 3.6%.The scavenging rate of DPPH and ABTS free radicals was 82.94%and 98.48%,the IC50 was 0.063 mg·mL^(-1) and 0.079 mg·mL^(-1),respectively.The total antioxidant capacity was 5.81μmol·mL^(-1).Conclusion The method is simple and repeatable,which can be used to determine the total flavonoids of Dracocephalum heterophyllum Benth,and the antioxidant activity of the total flavonoids of Dracocephalum heterophyllum Benth is good.

关 键 词：异叶青兰 总黄酮 含量测定 抗氧化 

分 类 号：R286[医药卫生—中药学]