Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping  

作　　者：Moosung Lee Kyoohyun Kim Jeonghun Oh YongKeun Park 

机构地区：[1]Department of Physics,Korea Advaneed In stitute of Science and Technology(KAIST),Daejeon 34141,South Korea. [2]KAIST Institute for Health Science and Technology,KAIST,Daejeon 34141,South Korea. [3]Tomocube Inc.,Daejeon 34109,South Korea. [4]Present address:Max Planck Institute for the Science of Light Max-Planck-Zentrum fur Physik und Medizin,91058 Erlangen,Germany

出　　处：《Light(Science & Applications)》2021年第6期1061-1069,共9页光（科学与应用)（英文版）

基　　金：KAIST UP programme,BK21+programme,Tomocube,and National Research Foundation of Korea(2017M3C1A3013923,2015R1A3A2066550,2018K000396).

摘　　要：A major challenge in three-dimensional(3D)microscopy is to obtain accurate spatial information while simultaneously keeping the microscopic samples in their native states.In conventional 3D microscopy,axial resolution is inferior to spatial resolution due to the inaccessibility to side scattering signals.In this study,we demonstrate the isotropic microtomography of free-floating samples by optically rotating a sample.Contrary to previous approaches using optical tweezers with multiple foci which are only applicable to simple shapes,we exploited 3D structured light traps that can stably rotate freestanding complex-shaped microscopic specimens,and side scattering information is measured at various sample orientations to achieve isotropic resolution.The proposed method yields an isotropic resolution of 230 nm and captures structural details of colloidal multimers and live red blood cells,which are inaccessible using conventional tomographic microscopy.We envision that the proposed approach can be deployed for solving diverse imaging problems that are beyond the examples shown here.

关 键 词：TRAPPING DETAILS RESOLVED 

分 类 号：TH742[机械工程—光学工程]