Functional Surfactants for Molecular Fishing,Capsule Creation,and Single-Cell Gene Expression  

作　　者：Mohammad Suman Chowdhury Xingcai Zhang Leila Amini Pradip Dey Abhishek Kumar Singh Abbas Faghani Michael Schmueck‑Henneresse Rainer Haag 

机构地区：[1]Institut Für Chemie Und Biochemie,Freie Universität Berlin,Takustrasse 3,14195 Berlin,Germany [2]John A.Paulson School of Engineering and Applied Sciences,Harvard University,Cambridge,MA 02138,USA [3]School of Engineering,Massachusetts Institute of Technology,Cambridge,MA 02139,USA [4]Berlin Institute of Health-Center for Regenerative Therapies,CharitéUniversitätsmedizin Berlin-CVK,Föhrer Str.15,13353 Berlin,Germany [5]BerlinCenter for Advanced Therapies,CharitéUniversitätsmedizin Berlin-CVK,Föhrer Str.15,13353 Berlin,Germany

出　　处：《Nano-Micro Letters》2021年第9期377-385,共9页纳微快报（英文版）

基　　金：This work was funded by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)-Project ID 387284271-SFB 1349 Fluorine-Specific Interactions

摘　　要：Creating a single surfactant that is open to manipulation,while maintaining its surface activity,robustness,and compatibility,to expand the landscape of surfactant-dependent assays is extremely challenging.We report an oxidation-responsive precursor with thioethers and multiple 1,2-diols for creating a variety of functional surfactants from one parent surfactant.Using these multifunctional surfactants,we stabilize microfluidics-generated aqueous droplets.The droplets encapsulate different components and immerse in a bioinert oil with distinct interfaces where an azide-bearing surfactant allow fishing of biomolecules from the droplets,aldehyde-bearing surfactant allow fabrication of microcapsules,and hydroxyl-bearing surfactants,with/without oxidized thioethers,allow monitoring of single-cell gene expression.Creating multifunctional surfactants poses opportunities for broad applications,including adsorption,bioanalytics,catalysis,formulations,coatings,and programmable subset of emulsions.

关 键 词：Dendronized fluorosurfactants Droplet microfluidics MICROCAPSULES Oxidation-responsive fluorosurfactants Cell encapsulation 

分 类 号：TQ423[化学工程]