高效油脂降解混合菌株筛选及降解条件的优化  

Screening of High-efficiency Oil-degrading Mixed Strains and Optimization of Degradation Conditions

作　　者：杨智博 李晓红 张丹丹[3] 辛蕊 翟訢宇 肖慧[1,2,3] YANG Zhibo;LI Xiaohong;ZHANG Dandan;XIN Rui;ZHAI Xinyu;XIAO Hui(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;College of life sciences,Zaozhuang University,Zaozhuang 277100,China;State Key Joint Laboratory of Environmental Simulation and Pollution Control,School of Environment,Tsinghua University,Beijing 100089,China;School of Marine Science and Technology,Tianjin University,Tianjin 300072,China;Institute of oceanology,Chinese academy of science Key laboratory of marine ecology&environmental science,CAS,Qingdao 266071,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]枣庄学院生命科学学院,山东枣庄277100 [3]清华大学环境模拟与污染控制国家重点联合实验室,北京100089 [4]天津大学海洋科学与技术学院,天津300072 [5]中国科学院海洋研究所,山东青岛266071

出　　处：《水处理技术》2021年第7期36-41,共6页Technology of Water Treatment

基　　金：中国海洋大学本科生研究发展计划(OUC-SRDP)项目(1412010517)。

摘　　要：从采自青岛台东步行街下水道的地沟油中分离可培养微生物,采用透明圈法筛选能够高效降解油脂的菌株,进行组合研究其油脂降解力,并对菌株进行形态学、生理生化特征和分子生物学鉴定。结果表明,分离到的菌株T5、C25和N30对油脂的降解能力较强,通过18S rDNA的方法鉴定菌株T5与地丝菌(Geotrichum fermentans)同源性最高达到97%。利用16S rDNA法鉴定菌株C25与假单胞菌(Pseudomonas sp.ALEB 33)同源性最高达到99%、菌株N30与鞘脂单胞菌(Sphingomonas sp.KA1)相似性达到99%。该菌株组合的适合pH和温度范围较广,适宜pH和温度分别为7和30℃,降解率最高达94.79%。可为油脂降解菌株混合组的构建和应用提供实验基础和依据。The culturable microorganisms were separated from the waste oil collected from the sewer of Taitung Pedestrian Street in Qingdao and the oil-degrading strains were screened by transparent circle method then their oil-degrading abilities were studied in combination. The strains were finally identified by morphology, physiological and biochemical characteristics and molecular biology. The results showed that, the separated strains T5, C25 and N30 had strong abilities to degrade oil. Using 18 S rDNA method to identify strain T5 had the highest homology of 97% with Geotrichum fermentans. Using 16 S rDNA method to identify train C25 had the highest homology of 97% with 99% with Pseudomonas sp. ALEB 33 and the similarity between strain N30 had Sphingomonas sp. KA1 reached 99%. This strain combination had a wide tolerance of suitable pH and temperature,the optimal pH and temperature was 7 and 30 °C, the degradation rate was up to 94.79%, which provided an experimental basis for the construction and application of a mixed group of oil-degrading strains.

关 键 词：油脂降解菌 筛选 鉴定 混合菌株 适宜条件 

分 类 号：X703.1[环境科学与工程—环境工程]