低剂量亚砷酸[As(Ⅲ)]和卷烟烟气总粒相物联合作用对人肺癌A549细胞氧化应激的影响  

Effect of low dose arsenious acid combined with total particulate matter of cigarette smoke on oxidative stress in human lung cancer cell line A549 cells

作　　者：赵俊伟[1] 尚平平[1] 华辰凤 李翔[1] 谢复炜[1] ZHAO Junwei;SHANG Pingping;HUA Chenfeng;LI Xiang;XIE Fuwei(Zhengzhou TobaccoResearch Institute of China National Tobacco Corporation,Zhengzhou 450001,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001

出　　处：《公共卫生与预防医学》2021年第4期27-30,共4页Journal of Public Health and Preventive Medicine

基　　金：中国烟草总公司郑州烟草研究院院长科技发展基金(322016CA0250)。

摘　　要：目的研究低剂量亚砷酸[As(Ⅲ)]和卷烟烟气总粒相物(total particulate matter,TPM)联合作用对人肺癌A549细胞氧化应激的影响。方法将人肺癌A549细胞分为4组:阴性对照组(含0.75%DMSO)、低剂量As(Ⅲ)单独作用组(0.88μg/mL,含0.75%DMSO)、卷烟烟气TPM单独作用组(75μg/mL)和联合作用组[TPM和As(Ⅲ)的浓度分别为75、0.88μg/mL],染毒24 h后,采用ELISA法检测细胞上清液中超氧化物歧化酶(superoxide dismutase,SOD)和细胞内8-羟基脱氧鸟苷(8-hydroxy-2-deoxyguanosine,8-OHdG)的含量水平,采用荧光探针DCFH-DA检测细胞内活性氧(reactive oxygen species,ROS)含量,采用2×2析因设计研究两者的交互作用。结果与对照组比较,联合作用组SOD浓度升高,差异有统计学意义(P<0.05);联合作用组、卷烟烟气TPM单独作用组ROS含量均升高,差异均有统计学意义(P<0.05);联合作用组、低剂量As(Ⅲ)单独作用组8-OHdG浓度均升高,差异均有统计学意义(P<0.05)。析因分析结果表明,低剂量As(Ⅲ)和卷烟烟气TPM联合作用对SOD、ROS及8-OHdG浓度的影响均存在交互作用,对SOD、ROS的影响为协同作用,对8-OHdG的影响为拮抗作用。结论低剂量亚砷酸[As(Ⅲ)]和卷烟烟气TPM联合作用对肺癌A549细胞氧化应激的影响存在交互作用。Objective To investigate the effect of low-dose arsenious acid solution(As(Ⅲ)) combined with total particulate matter(TPM) from cigarette smoke on cellular oxidative stress in human lung cancer cell line A549 cells. Methods A549 cells were divided into four groups: negative control group(0.75% DMSO),low dose As(Ⅲ) group(0.88% μg/mL,75% DMSO),cigarette smoke TPM group(75 μg/mL),and combined exposure group(75 μg/mL TPM,0.88 μg/mL As(Ⅲ)).After 24 hours′ exposure, the superoxide dismutase(SOD) level in cell culture medium and intracellular 8-hydroxy-2-deoxyguanosine(8-OHdG) content were detected by ELISA,and intracellular reactive oxygen species(ROS) level was detected by fluorescent probe DCFH-DA.2×2 factorial design was used to evaluate the interaction. Results Compared with the control group, the level of SOD in the combined exposure group was significantly increased(P<0.05).In addition, the ROS content in the combined exposure group and TPM alone group was significantly increased(P<0.05).The levels of 8-OHdG in the combined exposure group and low-dose As(Ⅲ) treated group were significantly higher than those in the control group(P<0.05).The results of the factorial analysis showed that low-dose As(Ⅲ) and TPM had interaction on SOD levels, ROS and 8-OHdG contents in A549 cells.The effects on SOD and ROS were synergistic, while the effect on 8-OHdG was antagonistic. Conclusion Low-dose arsenious acid solution As(Ⅲ) and TPM in cigarette smoke have interaction on oxidative stress in A549 cells.

关 键 词：亚砷酸[As(Ⅲ)] 总粒相物 氧化应激 交互作用 

分 类 号：R126[医药卫生—环境卫生学]