基于罗丹明B的光“关-开”型Fe3+荧光探针的构建研究  

Construction of a Highly Selective “off-on” Type Fe-fluorescent Probe Derived from Rhodamine B

作　　者：代婉婷 张军[1] 杨梅 刘昂 李琳 于春伟[1] DAI Wan-ting;ZHANG Jun;YANG Mei;LIU Ang;LI Lin;YU Chun-wei(a.School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou 571199,China;School of PublicHealth,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医学院热带医学与检验医学院,海南海口571199 [2]海南医学院公共卫生学院,海南海口571199

出　　处：《化学试剂》2021年第7期963-967,共5页Chemical Reagents

基　　金：国家自然科学基金资助项目(81860381,81760387);海南省自然科学基金高层次人才项目(820RC626);大学生创新性课题项目(S202011810015)。

摘　　要：过渡金属离子Fe3+在生命体系的发展过程中担当重要角色,荧光探针因其具有操作简便、灵敏度高、时空分辨能力强等特点得到了广泛应用。自主设计合成了一类新型的Fe^(3+)荧光探针,详细考查了其对不同金属离子的光谱分辨能力,结果表明在中性乙醇-水溶液中,探针只对Fe^(3+)表现出选择性的荧光增强和颜色变化(从无色到粉色),检测限达10^(-7) mol/L水平,这一变化是源于从内酰胺环(无荧光/off型)到开环胺(荧光/on型)的转化过程,对其他金属离子没有反应。利用等摩尔连续法测得探针与Fe3+的结合比为1∶1,同时EDTA实验证明该结合模式具有可逆性。The transition metal ion Fe3+ plays an important role in the development of living systems.Fluorescence probe is widely used because of its characteristics of simple operation, high sensitivity and strong spatial and temporal resolution ability.A fluorescent probe for sensing Fe3+ was designed and synthesized based on rhodamine B.It can selectively recognize Fe^(3+) with a detection limit down to 10^(-7 )mol/L,whereas no significant spectral changes were observed toward other metal ions in ethanol-water solution at pH 6.5.Fe^(3+)-specific binding would form the opening spirolactam ring, and consequently caused the appearance of the strong absorption at visible range accompanied by an obvious and characteristic color from colorless to pink.Furthermore, the 1∶1 binding mode was proposed based on Job′s method.Meanwhile, the coordination mode was reversible, as investigated by EDTA experiment.

关 键 词：FE3+ 罗丹明B 荧光探针 荧光增强 

分 类 号：O655.9[理学—分析化学]