基于SSR标记的小粒大豆品种(系)遗传多样性分析  

Genetic Diversity Analysis of Small Seed Soybean Varieties(Lines) Based on SSR Markers

作　　者：卢婧妍 胡刘涛 赵洪锟[2] 刘晓冬[2] 袁翠平[2] 齐广勋[2] 王玉民 董英山[2] LU Jingyan;HU Liutao;ZHAO Hongkun;LIU Xiaodong;YUAN Cuiping;QI Guangxun;WANG Yumin;DONG Yingshan(College of Agronomy,Jilin Agricultural University,Changchun 130118;Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林省农业科学院,长春130033

出　　处：《东北农业科学》2021年第3期27-33,共7页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程项目(CXGC2017ZD014);吉林省农业科技创新工程人才基金(C82230415)。

摘　　要：为了解东北地区小粒大豆品种(系)的遗传多样性特点,利用21对SSR引物对来自东北不同地区来源的44份小粒大豆品种(系)进行遗传多样性分析。结果表明,供试的44份东北地区小粒大豆品种(系)共检测到115个等位变异,平均每个位点等位变异数5.5个,等位变异数在3~11个之间,多态性信息含量指数(PIC)在0.309 1~0.815 7之间,平均为0.629 7。利用6对高多态性的SSR引物构建了44份小粒大豆品种(系)的指纹图谱,可以有效区分44份小粒大豆材料。44份小粒大豆品种(系)间的遗传相似系数变异范围为0.25~0.95,聚类分析结果表明44份小粒大豆材料可以分为5个类群,可以反映不同来源的小粒大豆品种(系)间的亲缘关系,可为小粒大豆遗传改良提供参考。To determine the genetic diversity characteristics small seed soybean varieties(lines) in Northeast China,21 pairs of SSR primers were used to analyze the genetic diversity of 44 small seed soybean varieties(lines) which were collected from different regions in Northeast China. The results showed that a total of 115 alleles were detected from 44 small seed soybean varieties(lines) in Northeast China and 3~11 alleles were detected at different SSR locus with an average of 5.5 alleles per SSR locus. The PIC ranged from 0.309 1 to 0.815 7 with an average of 0.629 7.Six pairs of highly polymorphic SSR primers were used to construct the fingerprints of 44 small seed soybean varieties(lines), which could discriminate these 44 small seed soybeans efficiently. Pair-wise genetic similarity coefficients of 44 small seed soybean varieties(lines) ranged from 0.25 to 0.95. The cluster analysis showed that 44 small seed soybean varieties(lines) could be divided into five major groups, reflecting a certain genetic exchange between small seed soybean varieties(lines) from different regions.

关 键 词：小粒大豆 遗传多样性 SSR标记 

分 类 号：S565.1[农业科学—作物学]