野生小花南芥体内AsA-GSH循环对土壤Cd、Pb胁迫的响应  

Response of ascorbate-glutathione cycle in wild Arabis alpina L.to soil Cd and Pb stress

作　　者：刘梅 李祖然 张光群 王吉秀 祖艳群[1] LIU Mei;LI Zu-ran;ZHANG Guang-qun;WANG Ji-xiu;ZU Yan-qun(College of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学资源与环境学院,昆明650201 [2]云南农业大学园林园艺学院,昆明650201

出　　处：《农业资源与环境学报》2021年第4期558-569,共12页Journal of Agricultural Resources and Environment

基　　金：国家自然科学基金项目(41867055,41967049,41761073)。

摘　　要：为探讨植物在重金属胁迫下的防御机制,通过野外调查的方法,分析了云南省会泽县铅锌矿区三个采样区野生小花南芥(Arabisalpina L.var.parviflora Franch)体内抗坏血酸-谷胱甘肽(Ascorbate-glutathione,AsA-GSH)循环对土壤Cd、Pb胁迫的响应。结果表明:驰宏区、小马坪、三多多三个采样点的土壤Cd、Pb含量存在差异,驰宏区采样点土壤的Cd、Pb含量显著高于小马坪和三多多采样点,同时驰宏区小花南芥根长、根表面积、根体积和株高均较高。驰宏区采样点野生小花南芥的丙二醛(Malondialdehyde,MDA)、过氧化氢(H2O2)、抗坏血酸(Ascorbicacid,AsA)和还原型谷胱甘肽(Reducedglutathione,GSH)含量显著高于小马坪。小花南芥根和叶中抗坏血酸过氧化物酶(Ascorbateperoxidase,APX)和谷胱甘肽转硫酶(GlutathioneS-transferase,GST)活性随土壤Cd、Pb含量的升高而显著提高;驰宏区野生小花南芥根部的谷胱甘肽还原酶(Glutathionereductase,GR)和谷胱甘肽过氧化物酶(Glutathioneperoxidase,GPX)活性显著低于小马坪。综上所述,在土壤Cd、Pb污染条件下,野生小花南芥通过体内AsA-GSH循环保持较强的抗氧化物质再生能力,增强对Cd、Pb的耐性。A field investigation was conducted to explore the response of ascorbate-glutathione(AsA-GSH)cycle in wild Arabis alpina L.var.parviflora Franch to soil Cd and Pb stress in three sampling sites in the Huize Pb-Zn mine area,Yunnan Province,China.The results showed that there were differences in the soil Pb and Cd contents in the three sites.The contents of Cd and Pb in the soil of Chihong site(CHQ)were significantly higher than those in the soil of Xiaomaping site(XMP)and Sanduoduo site(SDD).The root length,root surface area,root volume,and plant height of A.alpina were the highest in CHQ.The malondialdehyde(MDA),hydrogen peroxide(H2O2),AsA,and reduced GSH contents of wild A.alpina in CHQ were significantly higher than those in XMP.Ascorbate peroxidase(APX)and glutathione S-transferase(GST)activities in the roots and leaves increased significantly with increase in soil Cd and Pb contents;The glutathione reductase(GR)and glutathione peroxidase(GPX)activities of wild A.alpina roots in CHQ were significantly lower those in XMP.In summary,wild A.alpina can maintain a strong antioxidant capacity through the AsA-GSH cycle and enhance its tolerance to Cd and Pb stress in Cd and Pb polluted soil.

关 键 词：CD PB 小花南芥 AsA-GSH循环 

分 类 号：X53[环境科学与工程—环境工程] X173