小麦Brock抗白粉菌侵染早期防御应答基因分析  

Expression profiling of the early response genes against powdery mildew in resistant wheat Brock

作　　者：张露露 卞云迪 张驰 刘晓颖[1,2] 范宝莉[1,2] 王振英[1,2] ZHANG Lulu;BIAN Yundi;ZHANG Chi;LIU Xiaoying;FAN Baoli;WANG Zhenying(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2021年第3期40-46,共7页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31071671);天津市科技支撑资助项目(18YFZCNC01100,17JCZDJC34100).

摘　　要：为了探究小麦对禾本科布氏白粉菌的防御机制,以强抗白粉病小麦品种Brock为研究对象,利用RNA-seq技术分析了白粉菌侵染早期Brock中的应答基因,共鉴定出4625个差异表达基因(DEGs).利用KEGG分析重点注释了小麦与白粉菌互作的20条主要途径,通过qRT-PCR技术检测了来自不同途径的9个差异表达基因.其中,PR1和RPM1基因的表达量变化明显.这2个基因是PTI和ETI反应的重要参与基因,PR1表达量在2 hpi时显著升高,RPM1基因表达量在12 hpi时显著升高.以上结果说明白粉菌侵染早期(2 hpi和12 hpi),小麦通过PTI和ETI的协同作用抵抗白粉菌侵染.In order to explore the defense mechanism of wheat against Blumeria graminis f.sp.tritici(Bgt),the early response genes of the resistant wheat cultivar Brock were analyzed by RNA-seq technique.A total of 4625 differential expression genes(DEGs)were identified and major enriched in 20 wheat-Bgt interaction pathways of KEGG annotation.The expression levels of nine DEGs from different pathways had been detected by qRT-PCR,and the expression of PR1 and RPM1 changed significant-ly.They are two important genes involved in PTI and ETI.The expression level of PR1 increased obviously at 2 hpi,and RPM1 increased significantly at 12 hpi.The results showed that in the early stage of powdery mildew infection(2 hpi and 12 hpi),wheat is resistant to the infection through the synergistic effect of PTI and ETI.

关 键 词：白粉病 RNA-Seq技术 抗病小麦Brock 防御应答 

分 类 号：Q945.8[农业科学—植物病理学]