水稻早抽穗突变体zc1的表型分析及候选基因鉴定  

Phenotypic analysis and candidate gene identification of early-heading mutant zc1 in rice

作　　者：郝宏姣 王鑫 田瑶[1,2] 兰志鹏 周诗晨 刘晓男 郑瑞 王馨翊 张泗举 栾维江[1,2] HAO Hongjiao;WANG Xin;TIAN Yao;LAN Zhipeng;ZHOU Shichen;LIU Xiaonan;ZHENG Rui;WANG Xinyi;ZHANG Siju;LUAN Weijiang(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2021年第3期47-52,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31770343);天津市水稻产业技术体系研究计划资助项目(ITTRRS2018006);天津市自然科学基金重点资助项目(17JCYBJC30000).

摘　　要：从水稻T-DNA插入突变体库中筛选了一个早抽穗突变体zc1.为了克隆突变基因,对突变体株系进行了遗传分析,发现早抽穗表型和T-DNA共分离.利用热不对称交错PCR技术,扩增了T-DNA的侧翼序列.经序列比对发现,T-DNA插入到了一个富亮氨酸类受体激酶(LRR-RLK)基因的外显子区,造成了基因功能的丧失.候选基因的克隆,为进一步解析水稻抽穗期形成的分子机理奠定了基础.An early-heading mutant zc1 was screened from the rice T-DNA insertion mutant library.In order to clone the mutation gene,genetic analysis was carried out.The results showed that the early-heading phenotype was co-segregated with T-DNA.The flanking sequence of T-DNA was amplified by Tail-PCR,and it was found that the T-DNA inserted into the first exon of a LRR-RLK encoding gene,which resulted in the knockout of the gene.The cloning of candidate gene laid a foundation for further understanding the molecular mechanism of heading-date regulation in rice.

关 键 词：水稻 抽穗期 热不对称交错PCR 类受体蛋白激酶 

分 类 号：Q943[生物学—植物学]