Comparative Study of the Genetic Diversity, Antimicrobial Resistance, and Pathogenicity of Aeromonas Isolates from Clinical Patients and Healthy Individuals  

作　　者：MENG Shuang DU Xiao Li WANG Yong Lu QU Feng Tian XIE Gui Lin ZHOU Hai Jian HU Jin Rui QIN Zheng WANG Yue KAN Biao CUI Zhi Gang 

机构地区：[1]State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [2]Ma'anshan Center for Disease Control and Prevention,Ma'anshan 243000,Anhui,China [3]College of Life Science,Northeast Agricultural University,Ha'erbin 150000,Heilongjiang,China [4]School of Public Health,Jiamusi University,Jiamusi 154000,Heilongjiang,China

出　　处：《Biomedical and Environmental Sciences》2021年第6期454-464,共11页生物医学与环境科学（英文版）

基　　金：supported by Major Project of the thirteenth Five Year Special for infectious diseases of China[2018ZX10101002]。

摘　　要：Objective This study was performed to compare the genetic diversity,virulence,and antimicrobial resistance of Aeromonas strains isolated from patients and healthy individuals.Methods A total of 38 clinical strains and 19 strains from healthy individuals were isolated from the samples collected in Ma’anshan City,Anhui Province.Their taxonomy was investigated using concatenated gyrB-cpn60 sequences,and their resistance to 12 antibiotics was evaluated.The pathogenicity of these strains was examined through beta-hemolysis,protease activity,and virulence gene assays.Results The 57 Aeromonas strains were divided into 55 sequence types.Of these types,21 were novel,suggesting that their genetic diversity was high.These Aeromonas isolates could be divided into 7 species,and the positive rates of beta-hemolysis and protease activity were 49.1%and 73.7%,respectively.The detection rate of clinical patients in terms of beta-hemolysis and protease activity was higher than that of healthy individuals.Among the four most common Aeromonas strains,A.dhakensis had the highest detection rate of virulence genes.The multidrug resistance rate of the clinical isolates was much higher than that of the strains isolated from healthy individuals.Conclusions The taxonomy,virulence properties,and antibiotic resistance of Aeromonas isolates from patients differ from those of the isolates from healthy individuals.

关 键 词：AEROMONAS Genetic diversity Multidrug resistance Virulence gene Antimicrobial resistance gene 

分 类 号：R446.5[医药卫生—诊断学]