Tiaobu Feishen therapy inhibits inflammation induced by cigarette smoke extracts in a human monocyte/macrophage cell line  

作　　者：QIN Yanqin CHEN Yulong ZHAO Peng FENG Suxiang WU Yaosong LIU Xuefang DONG Haoran ZHENG Wanchun MAO Xiaoning LI Jiansheng 

机构地区：[1]Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine,Zhengzhou 450046,China [2]Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of China,Henan University of Chinese Medicine,Zhengzhou 450046,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第3期360-366,共7页中医杂志（英文版）

基　　金：Supported by National Natural Science Fund of China (No.81130062, 81403367);the National Key Technology R&D Program during the 12th Five-Year Plan Period(2014BAI10B06)。

摘　　要：OBJECTIVE: To study the mechanistic effects of Tiaobu Feishen therapy(TBFS) on inflammation induced by cigarette smoke extract(CSE) in a human monocyte/macrophage cell line.METHODS: The human monocyte/macrophage cell line THP-1 was stimulated with 10% CSE in the presence or absence of Bufei Yishen formula(BYF),Bufei Jianpi formula(BJF) and Yiqi Zishen formula(YZF). All formulations contained serum. Pro-inflammatory cytokines were measured in the supernatants using enzyme-linked immunosorbent assay.The activity of STAT3 DNA binding was detected using electrophoretic mobility shift assay and janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway activation was assessed using Western blotting.RESULTS: The results showed that BYF, BJF and YZF treatment strongly decreased the CSE-induced secretion of interleukin(IL)-6, IL-8, tumor necrosis factor-α and matrix metalloproteinase-9 by THP-1 cells. Furthermore, BYF, BJF and YZF treatment attenuated STAT3 DNA binding capacity and JAK2 and STAT3 were shown to be phosphorylated.CONCLUSION: The data revealed that BYF, BJF and YZF effectively inhibited a CSE-induced inflammatory response in THP-1 cells by limiting activation of the JAK2/STAT3 pathway.

关 键 词：cigarette smoking janus kinase 2 STAT3 transcription factor THP-1 cells matrix metalloproteinases Tiaobu Feishen therapy 

分 类 号：R563.9[医药卫生—呼吸系统]