Inhibitive efficacy of Nymphoides indica rhizome extract onα-glucosidase, and cross-link formation of advanced glycation end products  

作　　者：Muhammad Hanif Muzammil Ahmad Khan Muhammad Ramzan Abdul Rafey Ali Zaman Imran Aziz Ama'null'ah Adnan Amin 

机构地区：[1]Gomal Centre of Biochemistry and Biotechnology(GCBB),Gomal University D.I.Khan,29050,Pakistan [2]NPRL,Department of Pharmacognosy,Faculty of Pharmacy,Gomal University D.I.Khan,29050,Pakistan [3]FVAS,Gomal University D.I.Khan,29050,Pakistan

出　　处：《Journal of Traditional Chinese Medicine》2021年第3期376-380,共5页中医杂志（英文版）

摘　　要：OBJECTIVE: To investigate the inhibitive efficacy of Nymphoides Indica(L.) Kuntze rhizome extract onα-glucosidase and on cross-link formation of advanced glycation end products(AGEs).METHODS: The plant extracts were prepared by cold maceration and fractionated in solvents of diverse polarity. The in vitro α-glucosidase inhibition assay, fluorescence spectrometry and SDS-PAGE analysis was performed for antiglycation assays.RESULTS: During α-glucosidase inhibition assay significant inhibition by chloroform(0.43 mg/mL)and methanol fractions(0.66 mg/mL) was noticed.During the AGEs inhibition assay, both oxidative(BSA-MGO) and non-oxidative(BSA-glucose)modes were employed. The inhibition of AGEs by total extract was considered moderate(IC_(50)0.10 mg/mL) as a result of non-oxidative mode, whereas in case of oxidative mode(BASA-MGO) no activity was recorded. Among fractions the methanolic fraction presented significant results both in oxidative(IC_(50)0.01 mg/mL) and non-oxidative modes(IC_(50) 0.3 mg/mL). Likewise the ethyl acetate fraction was more active in non-oxidative mode(IC_(50)0.04 mg/mL) compared to oxidative mode(IC_(50)0.32 mg/mL).During assay for inhibition of cross-link formation,the chloroform fraction significantly inhibited cross-link formation in a dose dependent mode.CONCLUSION: It was finally concluded that N. Indica rhizome extract possesses significant properties that inhibit α-glucosidase, and AGEs cross-link formation.

关 键 词：Nymphoides Indica alpha-glucosidases glycation end products advanced protein cross links antiglycation 

分 类 号：R285[医药卫生—中药学]