预适应训练对高强度一次性力竭军事训练肾损伤大鼠的防护研究  

Protective effect of exercise preconditioning on kidney injury of rats caused by one-off high intensive and exhaustive military training

作　　者：陈洪 陈静[1] 胡婷婷 高丽丽[1] 林秀珠 张钰其 包晓辰 Chen Hong;Chen Jing;Hu Tingting;Gao Lili;Lin Xiuzhu;Zhang Yuqi;Bao Xiaochen(Cadre Health Care Department,the Sixth Medical Center of PLA General Hospital,Beijing 100037,China)

机构地区：[1]解放军总医院第六医学中心干部诊疗科,北京100037 [2]海军特色医学中心潜水与高气压医学研究室

出　　处：《中华航海医学与高气压医学杂志》2021年第3期360-364,369,共6页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：海军总医院创新培育基金(CXPY201832)。

摘　　要：目的通过构建高强度一次性力竭军事训练肾损伤模拟模型,观察不同强度、时长的预适应训练对大鼠早期肾损伤标志物的影响,为实施科学参训方案提供指导依据。方法70只雄性SD大鼠按随机数字表法分为7组,即高强度一次性军训肾损伤模型组(M组)、低强度短期预适应训练组(LS组)、低强度长期预适应训练组(LL组)、中强度短期预适应训练组(MS组)、中强度长期预适应训练组(ML组)、高强度短期预适应训练组(HS组)和高强度长期预适应训练组(HL组),每组10只。不同模式预适应跑台训练后按高强度一次性力竭军训肾损伤模型进行训练。观察大鼠每次训练结束后及力竭时行为特征,记录力竭时间,检测血清尿素氮(Bun)、肌酐(SCr)、血清胱抑素C(Cys C)、肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平和肾组织Na^(+),K^(+)-ATP酶活性,以及总超氧化物歧化酶(SOD)、丙二醛(MDA)水平和肾小管半定量评分。结果与其他组相比,HL组大鼠表情淡漠,反应迟钝,皮毛光泽度差,捕捉时逃避反应弱;其他组大鼠均皮毛润泽,对周围刺激反应灵敏。力竭时间从长至短排序为ML组、HS组、MS组、LL组、LS组、M组、HL组。与M组相比,LS组大鼠力竭时间差异无统计学意义(P>0.05);其他组组间比较,力竭时间有显著差异;HL组力竭时间明显短于其他各组,差异有统计学意义(P<0.05)。与M组比较,LS组大鼠血清Bun、SCr、Cys C、KIM-1、NGAL水平差异无统计学意义(P>0.05);其他组组间比较,大鼠血清Bun、SCr、Cys C、KIM-1、NGAL水平差异均有统计学意义(P<0.05)。各组大鼠肾组织Na^(+),K^(+)-ATP酶活性表达和总SOD活力从高到低排序为ML组、HS组、MS组、LL组、M组、LS组、HL组。与M组比较,LS组大鼠肾组织Na^(+),K^(+)-ATP酶活性表达和总SOD活力差异无统计学意义(P>0.05);其他组组间比较,大鼠肾组织Na^(+),K^(+)-ATP酶活性表达和总SODObjective To observe the effect of exercise preconditioning with different intensities and durations on early kidney injury indicators in rats by constructing a rat model of kidney injury induced by one-off high intensive and exhaustive military training,and to provide guidance on implementing scientific training plans.Methods A total of 70 male Sprague Dawley(SD)rats were divided into seven groups with 10 rats in each group according to the random number table method,namely,model group with kidney injury induced by one-off high-intensive military training(M group),low-intensive and short-term exercise preconditioning group(LS group),low-intensive and long-term exercise preconditioning group(LL group),medium-intensive and short-term exercise preconditioning group(MS group),medium-intensive and long-term exercise preconditioning group(ML group),high-intensive and short-term exercise preconditioning group(HS group),and high-intensive and long-term exercise preconditioning group(HL group).After treadmill exercise preconditioning in different modes,the rats were trained as the kidney injury model group receiving one-off high intensive and exhaustive military training.The behavioral characteristics of rats after each training and when they were trained to exhaustion,the time to exhaustion,the levels of blood urea nitrogen(Bun),serum creatinine(SCr),cystatin C(Cys-C),kidney injury molecule-1(KIM-1),and neutrophil gelatinase-associated lipocalin(NGAL),the activities of Na^(+),K^(+)-ATPase in kidney tissues,the levels of total superoxide dismutase(SOD),and malondialdehyde(MDA),and the semi-quantitative scores of renal tubules were observed.Results The rats in the HL group had indifferent expressions,slow responses,glossless fur,and weak responses for evading capture,while the rats in other groups had glossy fur and were alert to surrounding stimuli.In terms of the length of the time to exhaustion,the groups were ranked as ML>HS>MS>LL>LS>M>HL.There was no statistically significant difference in the time to exhaustion betw

关 键 词：预适应训练 肾损伤 大鼠 军事医学 

分 类 号：R828.1[医药卫生—临床医学]