Med19通过PTEN-PI3K/AKT信号通路影响卵巢癌细胞的生长  

Knockdown of mediator complex subunit 19 suppresses the growth of ovarian cancer via PTEN-PI3K/AKT signal pathway

作　　者：代东宇 朱雯博 王泽 李鑫蕊 蒋雨含 张丹[1] DAI Dong-yu;ZHU Wen-bo;WANG Ze;LI Xin-rui;JIANG Yu-han;ZHANG Dan(Department of Gynecology,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院妇科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2021年第3期244-247,共4页Journal of Harbin Medical University

基　　金：哈尔滨医科大学大学生创新创业训练项目(201910226171);黑龙江省卫生计生委科研课题(2018325)。

摘　　要：目的探究介导复合物19亚基(mediator coplex subunit 19,Med19)在卵巢癌中的作用及机制。方法通过慢病毒介导的RNAi抑制卵巢癌细胞中Med19的表达,采用免疫荧光及Western blot等方法检测抑制Med19后对卵巢癌细胞SKOV-3的影响。结果与对照组相比,转染siMed能够显著减少SKOV-3细胞中Med19 mRNA和蛋白的表达量(77%)(P<0.01),显著抑制SKOV-3细胞的增殖(55%)(P<0.01),并能够显著降低SKOV-3细胞中PTEN和磷酸化AKT的水平。此外,抑制AKT磷酸化显著降低了SKOV-3细胞中细胞分裂相关蛋白CyclinD1、CyclinB1及Survivin的表达量。结论 Med19能够在卵巢癌细胞中通过PETN及相关信号通路影响癌细胞的分裂。Objective To investigate the effect of the silencing of Med19 in human ovarian cancer SKOV-3 cells and its mechanism. Methods Lentivirus-mediated RNAi was employed to downregulate endogenous Med19 expression in SKOV-3 ovarian cancer cells. And the immunofluorescence and Western blot were used to investigate effects of Med19 on the SKOV-3 cells. Results Compared with the control group, the mRNA and protein expression levels of Med19 in siMed transfected-SKOV-3 cells were significantly decreased(77%)(P<0.01), the proliferation of siMed transfected-SKOV-3 cells was significantly inhibited(55%)(P<0.01), and the levels of PTEN and phosphorylated Akt in siMed transfected-SKOV-3 cells were significantly reduced. Besides, inhibition of AKT phosphorylation significantly reduced the expression levels of Cyclin1, CyclinB1 and Survivin which associated with cell division. Conclusion The proliferation of SKOV-3 ovarian cancer cells could be inhibited by Med19, which is might due to the effect of inhibition of AKT phosphorylation via PETN related signal pathway.

关 键 词：卵巢癌 增殖 MED19 PI3K/AKT 

分 类 号：R711.75[医药卫生—妇产科学]