槲皮素对脂多糖刺激下山羊瘤胃上皮细胞抗氧化和抗炎的影响  

Effects of quercetin on antioxidant and anti-inflammatory properties of goat rumen epithelial cells stimulated with lipopolysaccharide

作　　者：占今舜[1,2] 霍俊宏 詹康[3] 赵国琦[3] 武艳平[1] 马月辉[2] ZHAN Jinshun;HUO Junhong;ZHAN Kang;ZHAO Guoqi;WU Yanping;MA Yuehui(Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Science,Nanchang 330200,Jiangxi,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100093,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]江西省农业科学院畜牧兽医研究所,江西南昌330200 [2]中国农业科学院北京畜牧兽医研究所,北京100093 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《草业科学》2021年第7期1393-1401,共9页Pratacultural Science

基　　金：江西省现代农业产业技术体系建设专项(JXARS-13-肉羊岗位);江西现代农业科研协同创新专项(JXXTCX201702-04)。

摘　　要：为研究槲皮素对脂多糖(LPS)刺激下山羊瘤胃上皮细胞抗氧化和抗炎的影响,先将山羊瘤胃上皮细胞在添加0、5、10、20、40、60、80、120、160和320μg·mL^(−1)槲皮素的基础培养基中培养6 h后,通过检测细胞活性,确定80μg·mL^(−1)为槲皮素后续试验浓度。然后分成4组,山羊瘤胃上皮细胞在基础培养基(对照组,Con)和基础培养基[分别加入1μg·mL^(−1)的LPS(L)、80μg·mL^(−1)槲皮素(Q)以及1μg·mL^(−1) LPS和80μg·mL^(−1)槲皮素(L+Q)]中培养6 h后,测定相关指标。结果显示:1)与Con组相比,L组瘤胃上皮细胞的过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性显著降低(P<0.05),而丙二醛(MDA)浓度显著升高(P<0.05);Q组瘤胃上皮细胞的CAT、SOD、总抗氧化力(T-AOC)和谷胱甘肽过氧化物酶(GSH-PX)活性极显著升高(P<0.01),而MDA浓度显著降低(P<0.05)。与L组相比,L+Q组瘤胃上皮细胞的CAT、SOD、T-AOC和GSH-PX活性显著升高(P<0.05)。2)与Con组相比,L组瘤胃上皮细胞因子IK、趋化因子配体5(CCL5)、CXC趋化因子配体6(CXCL6)、CXCL8、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和IL-1β的mRNA相对表达量极显著升高(P<0.01)。与L组相比,L+Q组瘤胃上皮细胞IK、CCL5、CXCL6、CXCL8、IL-6、TNF-α和IL-1β的mRNA相对表达量极显著降低(P<0.01)。3)与Con组相比,L组瘤胃上皮细胞Toll样受体2(TLR2)、核转录因子κB(NF-κB)、髓样分化因子88(MyD88)和转录因子3(IRF3)的mRNA相对表达量显著升高(P<0.05),Q组瘤胃上皮细胞Toll样衔接蛋白(TOLLIR)和TLR 4的mRNA相对表达量显著降低(P<0.05)。与L组相比,L+Q组瘤胃上皮细胞TLR 2、NF-κB、MyD 88、TOLLIR和TLR 4的mRNA相对表达量显著降低(P<0.05)。综上所述,槲皮素能够提高山羊瘤胃上皮细胞的抗氧化和抗炎症性能,促进细胞增殖。The aim of this study was to investigate the effects of quercetin on the antioxidant and anti-inflammatory responses of goat rumen epithelial cells(GRECs)stimulated by lipopolysaccharide(LPS).GRECs were initially cultured in basal medium containing 0,5,10,20,40,60,80,120,160,and 320μg·mL^(−1) quercetin for 6 h.On the basis of the measurement of cell activity,a quercetin concentration of 80μg·mL^(−1) was used in subsequent experiments,which had the following four treatment groups:GRECs cultured in basal medium without quercetin or LPS(control group,Con);basal medium containing 80μg·mL^(−1) quercetin(Q);basal medium containing 1μg·mL^(−1) LPS(L);and basal medium containing 80μg·mL^(−1) quercetin and 1μg·mL^(−1) LPS(L+Q).Relevant indices of GRECs were determined after 6 h of culture.The following results were obtained.1)Compared with the control group,the activities of catalase(CAT)and superoxide dismutase(SOD)were significantly reduced in group L(P<0.05),whereas the content of malondialdehyde(MDA)was significantly increased(P<0.05).In group Q,compared with the control group,the activities of CAT,SOD,and glutathione peroxidase(GSH-PX),and total antioxidant capacity were significantly increased(P<0.01),whereas the content of MDA was significantly reduced(P<0.05).Compared with group L,the activities of CAT,SOD,and GSH-PX,and total antioxidant capacity were significantly increased in the L+Q group(P<0.05).2)Compared with the control group,relative mRNA transcript levels of the IK cytokines CCL5,CXCL6,CXCL8,IL-6,TNF-α,and IL-1βwere significantly elevated in group L(P<0.01),whereas compared with group L,the relative mRNA transcript levels of these six cytokines were significantly reduced in the L+Q group(P<0.01).3)Compared with the control group,the relative mRNA transcript levels of TLR 2,NF-κB,MyD 88,and IRF3 were significantly elevated in group L(P<0.05),whereas those of TOLLIR and TLR 4 were significantly reduced in group Q(P<0.05).Furthermore,compared with group L,the relative mRNA transcr

关 键 词：槲皮素 脂多糖 山羊 瘤胃上皮细胞 抗氧化性能 抗炎性能 细胞因子 

分 类 号：S827.5[农业科学—畜牧学]