Ragweed pollen induces allergic conjunctivitis immune tolerance in mice via regulation of the NF-κB signal pathway  

作　　者：Meng-Tian Bai Yun Li Zhu-Lin Hu 

机构地区：[1]Department of Ophthalmology,Fourth Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan Province,China [2]Yunnan Eye Institute,Kunming 650032,Yunnan Province,China [3]Key Laboratory of Yunnan Province for the Prevention and Treatment of Ophthalmology,Kunming 650032,Yunnan Province,China [4]Provincial Innovation Team for Cataract and Ocular Fundus Disease,Fourth Affiliated Hospital of Kunming Medical University,Kunming 650032,Yunnan Province,China [5]Expert Workstation of Yao Ke,Kunming 650032,Yunnan Province,China [6]Department of Ophthalmology,Suining Central Hospital,Suining 629000,Sichuan Province,China [7]Department of Oncology,Suining Central Hospital,Suining 629000,Sichuan Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2021年第7期955-964,共10页国际眼科杂志（英文版）

基　　金：Supported by the Provincial Innovation Team for Cataract and Ocular Fundus Disease in the Second People’s Hospital of Yunnan Province(No.2017HC010);the Key Laboratory of Yunnan Province for the Prevention and Treatment of Ophthalmology(No.2017DG008);Expert Workstation of Yao Ke(No.2017IC064)。

摘　　要：AIM:To investigate the feasibility and mechanism of immune tolerance in allergic conjunctivitis.METHODS:The allergic conjunctivitis immune tolerance mice model was established by ragweed pollen(RW)and the related cytokines were detected.The mice were divided into 9 groups and the maslinic acid(MA)or PBS were given for different group after modeling.The expression levels of chemokine ligand 5(CCL5)and P-65 in the conjunctival tissue were analyzed by immunohistochemistry,quantitative reverse transcription polymerase chain reaction(q RT-PCR)and Western blot.The percentage of interleukin-17(IL-17)and CD4+CD25+in the splenocyte supernatant was analyzed by flow cytometry.Fur thermore,the serum and splenocyte supernatant concentration of total-IgE,interleukin-10(IL-10),and IL-17 was analyzed by enzyme linked immune response(ELISA).RESULTS:After the model was established,symptoms of conjunctivitis were alleviated,the level of P-65,CCL5,IL-17,and total-IgE was raised,while the expression of IL-10,CD4+CD25+was decreased.This result fully demonstrated that a typical IL-17/regulatory-T-cells(Treg cells)imbalance and NF-κB activation.When the NF-κB signal pathway was suppressed,it showed that there was a further relief of conjunctivitis in mice.At the same time,the expression of total-IgE,IL-17,and CCL5 was decreased and the expression of anti-inflammatory factor(IL-10,CD4+CD25+)was increased.CONCLUSION:In the state of immune tolerance,symptoms of conjunctivitis in mice are alleviated,the Th-17 cells of allergic conjunctivitis mice are inhibited,and Treg cells activity is enhanced.

关 键 词：allergic conjunctivitis immune tolerance TH-17 cell Treg cell NF-κB signal pathway 

分 类 号：R285.5[医药卫生—中药学]