2019年云南省怒江州柯萨奇病毒A组16型的分子流行病学研究  

Molecular epidemiological analysis of coxsackievirus A16 in 2019 in Nujiang prefecture,Yunnan province of China

作　　者：罗定富 张丽芬[2] 徐敏 姜黎黎[2] 伏晓庆[2] 田炳均[2] LUO Ding-fu;ZHANG Li-fen;XU Min;JIANG Li-li;FU Xiao-qing;TIAN Bing-jun(Center for Disease Control&Prevention of Dehong Prefecture,Mangshi Yunnan 678400,China;不详)

机构地区：[1]德宏州疾病预防控制中心,云南芒市678100 [2]云南省疾病预防控制中心,云南昆明650022 [3]云南省科学技术院,云南昆明650000

出　　处：《中国病毒病杂志》2021年第3期168-171,共4页Chinese Journal of Viral Diseases

基　　金：国家“十三五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2017ZX10103010)。

摘　　要：目的了解2019年云南省怒江州柯萨奇病毒A组16型(coxsackievirus A16,CV-A16)毒株的分子流行病学特征,积累CV-A16的基因信息。方法对2019年云南省怒江州疾病预防控制中心手足口病实验室从手足口病患者中检测到的58株CV-A16毒株进行VP1区基因扩增、测序,采用Mega5.2软件和邻位相接法(neighbor-joining method)进行基因特征和分子流行病学分析。结果2019年云南省怒江州CV-A16毒株VP1区基因由891个核苷酸组成,共编码297个氨基酸。基因进化分析表明2019年怒江州58株CV-A16毒株中,57株(98.28%,57/58)为B1b亚型,1株(NJ134/YN/CHN/2019,简称NJ134)为B1c亚型(1.72%,1/58),未分离到B1a亚型和其他基因型。NJ134株与印度株的核苷酸一致性为95.30%~97.60%,氨基酸同源性为99.70%~100.00%,NJ134株最接近印度株,推测其来自印度。结论2019年云南省怒江州CV-A16毒株以B1b亚型为主,同时发现了1株B1c亚型,为国内首次发现。Objective To study the molecular epidemiology of coxsachievirus A16(CV-A16)from Nujiang prefecture of Yunnan province of China in 2019.Methods The VP1 gene of 58 strains of CV-A16 identified from Nujiang prefecture were amplified,sequenced and their genetic characteristics and molecular epidemiology were analyzed.Results The VP1 gene of CV-A16 was composed of 891 nucleotides(nt),coding for 297 amino acids(aa).Phylogenetic analysis showed that,of the 58 strains of CV-A16 from Nujiang prefecture in 2019,57 strains were B1 b subgenotype,accounting for 98.28%(57/58);one strain(NJ134-YN-CHN-2019,NJ134)was B1 c,accounting for 1.72%(1/58),no B1 a and other genotypes were identified.The nt and aa identity between NJ134 and the Indian B1 c strains were 95.30%-97.60%and 99.70%-100.00%,respectively.NJ134 was mostly closed to Indian strains.Conclusions B1 b subgenotype of CV-A16 caused HFMD occurred in Nujiang prefecture in 2019.Meanwhile,one strain of B1 c subgenotype was detected.This is the first report of B1 c subgenotype in China.

关 键 词：柯萨奇病毒A组16型(CV-A16) VP1基因 基因特征分析 分子流行病学 

分 类 号：R512.5[医药卫生—内科学]