单因素试验结合Box-Behnken设计-响应面法优化舒肝颗粒(广西方)的醇提工艺  

Optimization of Alcohol Extraction Technology of Shugan granules(Prescription of Guangxi)by Single Factor Experiment Combined with Box-Behnken Design-response Surface Methodology

作　　者：梁芳[1] 黄艳红[1] 龚志强[1] 蒙田秀[1] 吕潇 陈思宏 谭德慧 LIANG Fang;HUANG Yanhong;GONG Zhiqiang;MENG Tianxiu;LV Xiao;CHEN Sihong;Tan Dehui(Faculty of Chinese Medicine Science Guangxi university of Chinese medicine,Nanning 530222,China)

机构地区：[1]广西中医药大学赛恩斯新医药学院,广西南宁530222

出　　处：《中国民族民间医药》2021年第13期39-45,49,共8页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西中医药大学自然科学研究项目(2018MS012);国家级大学生创新创业训练计划项目(201913643015)。

摘　　要：目的:优化舒肝颗粒(广西方)的醇提工艺。方法:通过单因素试验筛选影响醇提工艺的关键因素,以乙醇体积分数、提取溶剂加入量、提取时间为考察因素,以提取液中迷迭香酸、虎杖苷、大黄素的总含量与浸膏得率的综合评分为评价指标,采用Box-Behnken设计-响应面法优化舒肝颗粒(广西方)的醇提工艺,并进行验证实验。结果:最佳醇取工艺条件为加入12倍量60%乙醇,提取2次,每次加热回流80 min。验证试验中3批样品提取后的综合评分为99.54%,与模型预测值98.16%相比较,相对误差为0.46%(RSD=0.40%,n=3)。结论:优化的醇提工艺合理可行,可为舒肝颗粒(广西方)的工艺研究提供参考。Objective To optimize the alcohol extraction technology of Shugan granules(Prescription of Guangxi).Methods Screening of key factors influencing the alcohol extraction process by single factor tests.Using volume fractions of alcohol,volume of addition and extraction time as factors,taking comprehensive scoring value of the total contents of rosmarinic acid,polydatin,emodin and the yield of extract as evaluation index,Box-Behnken design-response surface method was used to optimize the alcohol extraction technology of Shugan granules(Prescription of Guangxi).Validation test was also conducted.Results The optimal extraction technology was adding 12 times of 60%alcohol,extracting for 2 times,reflexing for 80min each time.In validation test,the average of comprehensive scoring value was 99.54%,the relative deviation of which to predicted value(98.16%)was 0.46%for 3 batches of samples(RSD=0.40%,n=3).Conclusions The optimized alcohol precipitation process for Shugan granules(Prescription of Guangxi)is rational and feasible,which can provide a reference for process studies in the future.

关 键 词：舒肝颗粒(广西方) 醇提工艺 总含量 Box-Behnken设计-响应面法 

分 类 号：R932[医药卫生—生药学]