HPLC测定小儿清热止咳口服液中苯甲酸的含量  

How to Measure the Coverage of Benzoic Acid in the Lnfant Heat and Cough Clearing Oral Liquid by HPLC

作　　者：尕藏卓玛 杨丽琼 达娃卓玛 GAZANG Zhuoma;YANG LiQiong;DAWA Zhuoma(Tibet Autonomous Region Institute for Food and Drug Control and Research,Lhasa 850000,China)

机构地区：[1]西藏自治区食品药品检验研究院,西藏拉萨850000

出　　处：《中国民族民间医药》2021年第13期50-53,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:用HPLC法测定全国范围30家生产企业57批次小儿清热止咳口服液中苯甲酸的含量。方法:Inertsustain C18(5μm,250 mm×4.6 mm)柱,柱温35℃;流动相:乙腈-0.02 mol/mL乙酸铵溶液(5∶95);流速:1.0 mL/min;检测波长:240 nm。结果:苯甲酸在6.1350~184.0488μg/mL线性关系良好(r>0.9998);平均回收率102.94%。结论:30家企业57批次样品均检出苯甲酸,且各企业样品中苯甲酸含量差异很大。Objective Using the HPLC to check the benzoic content in the children's oral liquid clearing heat and relieving cough in 30 enterprises.The specific method is,Inertsustain C18(5μm,250 mm×4.6 mm)column,column temperature is 35℃;mobile phase:acetonitrile-0.02 mol/ammonium acetate solution(5∶95);current velocity:1.0 mL/min;detection wavelength:230 nm.Result Benzoic acid shows great in the linear relation of 6.1350~184.0488μg/mL(r>0.9998);average recovery rate should be 102.94%.Conclusion we have checked the benzoic acid in 60 times of samples in 30 enterprises,and the benzoic acid content is different from different enterprises.

关 键 词：小儿清热止咳口服液 苯甲酸 测定 

分 类 号：R284.1[医药卫生—中药学]