二烯丙基硫化物在百草枯中毒大鼠肺组织中的作用  

The effect of diallyl sulfide on the lung tissue of rats poisoned by paraquat

作　　者：曹玉芳[1] 计超[1] 管军 余燕梅 宋维[2] Cao Yufang;Ji Chao;Guan Jun;Yu Yanmei;Song Wei(Department of Critical Care Medicine,Affiliated Haikou Hospital of Xiangya Medical College,Central South University,Haikou 570300,China;Emergency Center of Hainan Provincial People's Hospital,Haikou 570300,China)

机构地区：[1]中南大学湘雅医学院附属海口医院重症医学科,海口570300 [2]海南省人民医院急救中心,海口570300

出　　处：《中华劳动卫生职业病杂志》2021年第6期438-441,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：海南省自然科学基金(318MS133)。

摘　　要：目的探讨二烯丙基硫化物(DAS)对百草枯(PQ)中毒所致大鼠急性肺损伤的作用机制。方法于2016年5月,将成年清洁级雄性Wistar大鼠32只,按随机数字表法分为对照组、模型(PQ)组、DAS治疗组及地塞米松(DXM)治疗组,每组8只。一次性灌胃PQ溶液(70 mg/kg)制备PQ中毒大鼠模型;制模前后分别经腹腔注射DAS 100 mg/kg(DAS治疗组)、生理盐水(对照组和PQ组)及DXM 1 mg/kg(DXM治疗组)。24 h后处死大鼠,观察肺组织损伤程度并进行病理学评分;测定肺组织诱导型一氧化氮合酶(iNOS)表达;分离获取肺泡巨噬细胞并培养,取其上清液测定NO含量;测定大鼠肺泡巨噬细胞中iNOS mRNA表达。结果与对照组比较,PQ组大鼠肺组织病理损伤评分和iNOS表达明显增加,并且肺泡巨噬细胞分泌NO含量及iNOS mRNA表达明显增加,差异均有统计学意义(P<0.05)。与PQ组比较,DAS治疗组和DXM治疗组大鼠肺组织病理损伤评分和iNOS表达明显降低,肺泡巨噬细胞分泌NO含量及iNOS mRNA表达明显降低,差异均有统计学意义(P<0.05)。DXM治疗组和DAS治疗组间上述指标差异均无统计学意义(P>0.05)。结论DAS可能对PQ中毒大鼠的急性肺损伤具有保护作用。Objective To investigate the mechanism of diallyl sulfide(DAS)on paraquat(PQ)-induced acute lung injury in rats.Methods In May 2016,32 adult male Wistar rats were randomly divided into control group,model(PQ)group,DAS treatment group and dexamethasone(DXM)treatment group,with 8 rats in each group.PQ poisoning model was established by intragastric administration of PQ solution(70 mg/kg).100 mg/kg DAS(DAS treatment group),normal saline(control group and PQ group)and 1 mg/kg DXM(DXM treatment group)were injected intraperitoneally before and after modeling.After 24 hours,the rats were killed and the degree of lung injury was observed.The expression of inducible nitric oxide synthase(iNOS)in lung tissue was measured.Alveolar macrophages were isolated and cultured.The supernatant was taken to determine the content of NO,and the expressions of iNOS mRNA in alveolar macrophages were detected.Results Compared with the control group,the pathological injury score and the expression of iNOS in the lung tissue of PQ group were significantly increased,and the content of NO secreted by alveolar macrophages and the expression of iNOS mRNA were significantly increased(P<0.05).Compared with PQ group,the pathological injury scores and the expressions of iNOS in lung tissue of rats in DAS treatment group and DXM treatment group were significantly decreased,and the contents of NO secreted by alveolar macrophages and the expressions of iNOS mRNA were significantly decreased(P<0.05).There was no significant difference between DXM group and DAS group(P>0.05).Conclusion DAS may have protective effect on acute lung injury induced by PQ in rats.

关 键 词：百草枯 一氧化氮合酶 急性肺损伤 二烯丙基硫化物 肺泡巨噬细胞 大鼠 

分 类 号：R595.4[医药卫生—内科学]