海洋链霉菌OUCMDZ-3434 wailupemycins生物合成基因簇中调控基因功能研究  

Functional study of regulatory genes in wailupemycins biosynthetic gene cluster from the marine-derived Streptomyces sp.OUCMDZ-3434

作　　者：张玉峤 林筱钰 肖菲 李花月 朱伟明[1,2] 李文利 ZHANG Yu-qiao;LIN Xiao-yu;XIAO Fei;LI Hua-yue;ZHU Wei-ming;LI Wen-li(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2021年第3期22-28,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金委员会中泰国际合作项目(81561148012)资助。

摘　　要：目的对海洋链霉菌Streptomyces sp.OUCMDZ-3434中聚酮类化合物wailupemycins生物合成基因簇wal中调控基因walC与walV进行功能探究。方法通过生物信息学手段对walC和walV功能进行分析,采用PCR-targeting策略分别阻断walC和walV基因,高效液相色谱法(HPLC)分析野生株与突变株在相同条件下发酵产物的差异。结果生物信息学分析表明walC和walV分别编码TetR家族和MerR家族转录调控因子,成功获得阻断突变株Streptomyces sp.OUCMDZ-3434ΔwalC和Streptomyces sp.OUCMDZ-3434ΔwalV,HPLC分析发现2株阻断突变株中wailupemycin G产量未发生明显变化。结论walC和walV对wailupemycin类化合物的产生可能并未起到明显调控作用,该类化合物的生物调控机理有待进一步研究。Objective To investigate the function of two regulatory genes walC and walV in the polyketide wailupemycins biosynthesis gene cluster wal in Streptomyces sp.OUCMDZ-3434.Methods walC and walV were analyzed by bioinformatic analysis and knocked out respectively by using PCR-targeting strategy.The mutants and the wild-type strain were fermented under the same conditions,and the fermentation broths were analyzed by HPLC.Results Bioinformatic analysis showed wal C and wal V encoded a putative TetR family and MerR family transcriptional regulator,respectively.Streptomyces sp.OUCMDZ-3434ΔwalC and Streptomyces sp.OUCMDZ-3434ΔwalV mutants were constructed successfully.HPLC analysis suggested that the yield of wailupemycin G in Streptomyces sp.OUCMDZ-3434ΔwalC or Streptomyces sp.OUCMDZ-3434ΔwalV mutants did not change obviously.Conclusion wal C and wal V might not have obvious regulatory effects on the production of wailupemycins,and the biological regulation mechanism of these compounds needed further study.

关 键 词：海洋链霉菌 转录调控 天然产物 wailupemycins 

分 类 号：R915[医药卫生—微生物与生化药学]