EMT标志物在胎膜早破患者羊膜上皮细胞中的表达及意义  

Expression and significance of EMT markers in amniotic epithelial cells of patients with premature rupture of membranes

作　　者：陈静宁 许静芸 罗晓薇 张元鑫 吴盛桂 宫国良 CHEN Jingning;XU Jingyun;LUO Xiaowei;ZHANG Yuanxin;WU Shenggui;GONG Guoliang(Department of Gynecology and Obstetrics,Shantou Jinping Women and Children􀆳s Hospital,Shantou,Guangdong,China,515044;Department of Pathology,The First Affiliated Hospital of Shantou University Medical College,Shantou,Guangdong,China,515044;Department of Pathology,Chaonan Minsheng Hospital of Shantou,Shantou,Guangdong,China,515044)

机构地区：[1]汕头市金平区妇幼保健院妇产科,广东汕头515044 [2]汕头大学医学院第一附属医院病理科,广东汕头515044 [3]汕头潮南民生医院病理科,广东汕头515044

出　　处：《分子诊断与治疗杂志》2021年第6期857-860,共4页Journal of Molecular Diagnostics and Therapy

基　　金：2019年汕头市科技计划重点项目 汕府科【2019】106号。

摘　　要：目的探讨上皮-间叶转化(EMT)标志物E-钙粘附蛋白(E-cadherin)、波形蛋白(Vimentin)、基质金属蛋白酶9(MMP9)、基质金属蛋白组织抑制因子1(TIMP1)在胎膜早破(PROM)患者胎盘羊膜上皮细胞中的表达意义。方法 221例胎膜早破患者分为足月胎膜早破(TPROM)和早产胎膜早破(PPROM)两组,其中TPROM 143例,PPROM 78例,匹配同一时间段内正常足月产51例为对照组,收集患者的基本临床信息;应用免疫组织化学技术对胎盘羊膜组织中EMT标志蛋白E-cadherin、Vimentin、MMP9、TIMP1进行检测。结果与对照组比较,PPROM和TPROM组在孕、产次指标上,差异有统计学意义(P<0.05)。年龄、白细胞计数、红细胞计数、血红蛋白值、血小板计数、凝血酶时间、白蛋白、球蛋白等指标在三组间比较,差异无统计学意义(P>0.05);E-cadherin在各组间均出现阳性表达,以强表达为主,组间比较,差异无统计学意义(P>0.05),而Vimentin、MMP9、TIMP1在各组间出现明显的差异性表达,差异有统计学意义(P<0.05)。结论 EMT参与了PROM的发病过程,Vimentin、MMP9、TIMP1在羊膜上皮细胞中的表达可以作为发生EMT的标志物,在临床工作中,通过干预EMT这一病理过程,有望成为治疗胎膜早破的一个新思路。Objective To investigate the expression of E-cadherin, vimentin, matrix metalloproteinase-9(MMP-9)and tissue inhibitor of matrix metalloproteinase 1(TIMP1). Methods Two hundred and seventy-two patients with PROM were divided into a full-term PROM(TPROM)group(n=143),and a preterm PROM(PPROM)group(n=78),fifty-one women with normal term delivery in the same period were selected as a control group. The basic clinical information of the patients was collected first,and then the EMT marker proteins E-cadherin,vimentin,MMP9 and TIMP1 in the placental amniotic membrane tissue were detected by immunohistochemistry. Results Compared with the control group,the PPROM and TPROM groups had significant differences in pregnancy and birth order(P<0.05). There were no statistically significant differences in age,white blood cell count,red blood cell count,hemoglobin value,platelet count,thrombin time,albumin,and globulin among the three groups(P>0.05). E-cadherin was positively expressed in all groups,mainly with strong expression,and the comparison between groups showed no statistical significance(P>0.05). However,vimentin,MMP9,and TIMP1 were significantly differentially expressed in all groups,and the difference was statistically significant(P<0.05). Conclusion EMT is involved in the pathogenesis of PROM.The immunohistochemical method of detecting the expression of Vimentin,MMP9 and TIMP1 in amniotic epithelial cells can be used as markers for the occurrence of EMT. In clinical practice,intervention in the pathological process of EMT is expected to be a new idea for the treatment of premature rupture of membranes.

关 键 词：上皮-间叶转化 免疫组织化学 胎盘 羊膜 胎膜早破 

分 类 号：R714.433[医药卫生—妇产科学]