南京市1例暴露感染HIV病例的溯源调查  

Traceability investigation of a case of exposure and infection with HIV in Nanjing

作　　者：董潇潇[1] 许文炯[1] 乔梦凯[1] 毛子晴 张洪英[1] 王燕[1] 吴咏梅[1] DONG Xiaoxiao;XU Wenjiong;QIAO Mengkai;MAO Ziqing;ZHANG Hongying;WANG Yan;WU Yongmei(Nanjing Municipal Center for Disease Control and Prevention,Nanjing,Jiangsu,China,210003)

机构地区：[1]南京市疾病预防控制中心,江苏南京210003

出　　处：《分子诊断与治疗杂志》2021年第6期973-976,共4页Journal of Molecular Diagnostics and Therapy

基　　金：南京市医学科技发展一般性课题(YKK18176);南京市医学科技发展重点课题(ZKX19048)。

摘　　要：目的明确疑似医源性感染的医务人员与暴露源HIV感染病例之间的传播关系。方法采集疑似医源性感染者和暴露源病例的血样,提取血浆RNA,逆转录及巢式PCR扩增后测序,进行基因亚型分析,计算基因距离并构建系统进化树。结果疑似医源性感染的医务人员的HIV基因亚型为CRF-01AE重组亚型,暴露源病例为CRF-07BC重组亚型,两者pol区、gag区和env区的基因距离分别为0.135、0.187、0.319,均大于各自与其对应相似株的基因距离,差异有统计学意义(P<0.05)。结论认为该医务人员与其暴露源HIV感染病例不存在传播关系。Objective To clarify the transmission relationship between the medical staff suspected of iatrogenic infection and the source case of HIV infection. Methods Blood samples were collected from medical staff suspected of iatrogenic infection and the source patient of HIV infection. RNA was extracted from plasma,amplified by RT-PCR and nested PCR,and then sequenced for gene subtype analysis. Gene distance was calculated and phylogenetic tree was constructed. Results The HIV gene subtype of medical staff suspected of iatrogenic infection is CRF-01 AE recombinant subtype,and the exposure source case of is CRF-07 BC recombinant subtype. The genetic distances of the pol,gag and env regions were 0.135,0.187 and 0.319,respectively,which were greater than the genetic distances between each and their corresponding similar strains,and the difference was statistically significant(P<0.05). Conclusion It is considered that there is no transmission relationship between the medical staff and the HIV infection cases from which they were exposed.

关 键 词：艾滋病 职业暴露 分子溯源 

分 类 号：R512.91[医药卫生—内科学]