野豌豆属种子形态多样性与种子分类鉴定方法的研究  

Study on Seed Morphological Diversity and Seed Classification and Identification Method of Vicia

作　　者：刘博文 黎桂阳 常媛飞 金美燕 刘芳[3] 高秋[3] 刘万良 哈斯塔米尔 王显国[1] LIU Bo-wen;LI Gui-yang;CHANG Yuan-fei;JIN Mei-yan;LIU Fang;GAO Qiu;LIU Wan-liang;Hasitamier;WANG Xian-guo(College of Grassland Science Technology, China Agricultural University, Beijing 100193, China;Henan Green Engineering Co., Ltd, Zhengzhou, Henan Province 450000, China;National Livestock Station, Beijing 100125, China;Grassland Workstation of Forestry and Grassland Bureau of Ulanhot,Ulanhot, Inner Mongolia 137400, China;People’s Government of Shaogen Town, Ar Horqin Banner, Chifeng, Inner Mongolia 025567, China)

机构地区：[1]中国农业大学草业科学与技术学院,北京100193 [2]河南省绿色工程有限公司,河南郑州450000 [3]全国畜牧总站,北京100125 [4]乌兰浩特市林业和草原局草原工作站,内蒙古乌兰浩特137400 [5]阿鲁科尔沁旗绍根镇人民政府,内蒙古赤峰025567

出　　处：《草地学报》2021年第7期1375-1385,共11页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(31772657);国家牧草产业技术体系良种繁育项目资助。

摘　　要：本研究以48份野豌豆属的14个种为研究对象,以17个种子表型性状来揭示野豌豆属表型性状的遗传多样性,并对其进行分类鉴定。结果表明:种子表型性状种间变异较大,数量性状中百粒重变异系数最大,为81.92%,种形指数的Shannon多样性信息指数最大,为1.94;质量性状中种脐颜色的变异系数最大,为75.12%,其Shannon多样性信息指数也最大,为1.56;主成分分析表明前6个主成分累计贡献率达86.09%;相关性分析表明种子长、种子宽、种子厚和百粒重相互之间呈极显著正相关关系(P<0.01);在相似性系数为0.79时可将试验材料分为5大类群。本研究中百粒重、种脐颜色、种皮颜色、种皮纹理、种脐长/种子长、子叶颜色、胚根基部斑纹、种脐形状、胚根宽/胚根长可作为该14种野豌豆属植物种间分类鉴定依据,并据此制作相应检索表,为野豌豆属的鉴定提供依据。In this study,48 accessions of 14 species of Vicia were studied,17 seed phenotypic traits were used to reveal the genetic diversity of Vicia phenotypic traits,and to classify and identify them.The results showed that the phenotypic traits of seed had great variation among species,the coefficient of variation of hundred-grain weight in quantitative traits was the largest,which was 81.92%,and the Shannon diversity information index of the seed form index was the largest,which was 1.94.Among the quality traits,the umbilical color had the largest variation coefficient,which was 75.12%,and its Shannon diversity information index was also the largest,which was 1.56.The results of principal component analysis showed that the cumulative contribution rate of the first six principal components was 86.09%.The correlation analysis between quantitative traits showed that seed length,seed width,seed thickness and hundred-grain weight were extremely significantly positively correlated with each other(P<0.01),and they were also positively correlated with radicle length and umbilical length.The cluster analysis divided the 14 species of Vicia into five groups.According to the analysis of the phenotypic shape of Vicia,hundred-grain weight,hilum color,testa color,testa texture,hilum-seed ratio,cotyledon color,radicle base markings,hilum shape,radicle width/radicle length can be used as the basis for the classification and identification of the 14 species of Vicia,and the classification key can provide a basis for the identification of the Vicia.

关 键 词：野豌豆属 种子形态多样性 分类鉴定 

分 类 号：S541.9[农业科学—作物学] Q944-3[生物学—植物学]