草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析  

Cloning and Expression Analysis of NRT1/PTR FAMILY 8.3 Gene in Kentucky Bluegrass

作　　者：金一锋[1,2] 陈阳 齐欣[1] 高岩松 金忠民 赵清峰[1] 王琦 JIN Yi-feng;CHEN Yang;QI Xin;GAO Yan-song;JIN Zhong-min;ZHAO Qing-feng;WANG Qi(College of Life Science and Agro-Forestry, Qiqihar Univesity, Qiqihar, Heilongjiang Province 161006, China;Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar, Heilongjiang Province 161006, China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《草地学报》2021年第7期1397-1405,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金(31501785);黑龙江省普通高等学校青年创新人才培养计划项目(UNPYSCT-2018102);黑龙江省省属高等学校基本科研业务费科研项目(135309365);齐齐哈尔大学大学生创新创业项目(202010232161,202010232170,202010232802)资助。

摘　　要：植物硝酸盐转运蛋白(Nitrate transporter,NRT)可有效转运NO-3,提升氮素利用效率。为了解析草地早熟禾(Poa pratensis)适应不同浓度及不同形态氮素、干旱胁迫机理,本研究以草地早熟禾为试材,对NRT1/PTR FAMILY 8.3基因进行克隆、生物信息学分析,并分析了不同浓度及不同形态氮素以及干旱下该基因表达情况。研究结果:草地早熟禾NRT1/PTR FAMILY 8.3基因包含典型的主要协同转运蛋白超家族(Major facilitator superfamily,MFS)结构域,与二穗短柄草(Brachypodium distachyon)高度同源;荧光定量PCR分析表明,该基因根部、叶部的表达量显著高于茎部;氮浓度为7.5 mM时,不同形态氮素诱导下,NaNO3处理组有利于该基因表达;无氮、高氮组(0,15 mM NaNO3)基因的表达量显著高于适氮组(7.5 mM NaNO3);干旱胁迫可促进其表达。研究结果为探究NRT1/PTR FAMILY 8.3在不同氮素环境中的调节机制,培育氮素利用率高的优质草种提供理论基础。As the main part of urban landscaping,lawn plays an important role in improving the ecological environment.Poa pratensis L.is a cool-season turfgrass with high quality.Poa pratensis L.has a high demand for nitrogen nutrients.Nitrate transporter(NRT)in plants can effectively transport NO-3 and improve nitrogen-use efficiency.In this study,the NRT1/PTR FAMILY 8.3 gene of Midnight II was cloned and analyzed its bioinformation and expression under different nitrogen concentrations,nitrogen forms and drought treatments.The main results showed that the NRT1/PTR FAMILY 8.3 gene was cloned from Poa pratensis L.,which belonged to the major facilitator superfamily(MFS)and was highly homologous to the NRT1/PTR FAMILY 8.3 gene from Brachypodium distachyon.qRT-PCR results showed that the NRT1/PTR FAMILY 8.3 gene was highly expressed in roots and leaves of Poa pratensis L.After induction with different nitrogen forms at the same concentration,the expression of NRT1/PTR FAMILY 8.3 gene in the NaNO3 treatment group was the highest.The expression of NRT1/PTR FAMILY 8.3 gene under nitrogen starvation and high-concentration nitrate nitrogen(0,15 mM NaNO3)was higher compared with that under suitable nitrogen(7.5 mM NaNO3).Drought stress promoted the expression of NRT1/PTR FAMILY 8.3 gene to a certain extent.The cloning and expression analysis results of Poa pratensis L.NRT1/PTR FAMILY 8.3 provided a theoretical basis for further exploring its regulatory mechanism in different nitrogen environments,determining its affinity to nitrate nitrogen,and cultivating high-quality turfgrass varieties with high nitrogen-use efficiency.

关 键 词：草地早熟禾 NRT1/PTR FAMILY 8.3 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学]