两种生长阶段的牛蛙蝌蚪对盐度的耐受性研究  

Study on the Tolerance to Salinity of Tadpole of Rana catesbeiana Shaw at Two Growth Stages

作　　者：梁家豪 鲜雪梅 LIANG Jia-hao;XIAN Xue-mei(College of Resources and Environment,Aba Teachers University,Wenchuan,Sichuan 623002)

机构地区：[1]阿坝师范学院资源与环境学院,四川汶川623002

出　　处：《安徽农业科学》2021年第14期85-88,97,共5页Journal of Anhui Agricultural Sciences

基　　金：阿坝师范学院青年基金项目(ASC20-04);国家级大学生创新训练项目(202010646031);2019年学生科研项目(201910089,201910099);阿坝师范学院质量工程项目(201909023)。

摘　　要：在人工养殖条件下,盐度常作为影响蝌蚪生长发育的环境因子之一。在实验室温度(23.1±2.0)℃环境下,采用单因子静态急性毒性试验法研究2种生长期牛蛙(Rana catesbeiana Shaw)蝌蚪对水体盐度的耐受能力。结果表明,在试验条件水质下,处于25期牛蛙蝌蚪及五趾分化期牛蛙蝌蚪的24、48、72和96 h半致死浓度分别为6.41、5.44、5.50、5.37 g/L及12.56、10.48、7.67、4.09 g/L,安全值分别为1.18和2.19 g/L。在相同条件下,五趾分化期牛蛙蝌蚪比25期牛蛙蝌蚪对盐度的耐受能力更强。建议在人工养殖过程中,将25期牛蛙蝌蚪及五趾分化期牛蛙蝌蚪分池饲养,二者长期生存的安全盐度控制在1.3 g/L以下。Under the conditions of artificial culture,salinity was often used as one of the environmental factors that affected the growth and development of tadpoles.The tolerance to salinity of tadpoles of Rana catesbeiana Shaw at two growth stages was studied by using a single-factor static acute toxicity test under the laboratory environment with an average temperature of(23.1±2.0)℃.The results showed that under the experimental conditions,24,48,72 and 96 h LC 50 of R.catesbeiana tadpoles at the 25 stage and 5-toed differentiation stage were 6.41,5.44,5.50,5.37 and 12.56,10.48,7.67,4.09 g/L respectively,SC value was 1.18 and 2.19 g/L respectively.Under the same conditions,the tolerance to salinity of R.catesbeiana tadpole at 5-toed differentiation stage was stronger than that of bullfrog tadpole at the 25 stage.It was suggested that in the process of artificial breeding,R.catesbeiana tadpoles at 25-stage and 5-toed differentiation stage should be fed in separate ponds,and the safe salinity of their long-term survival should be controlled below 1.3 g/L.

关 键 词：牛蛙蝌蚪 半致死浓度 盐度 耐受性 安全值 

分 类 号：S917.4[农业科学—水产科学]