向日葵抗列当基因Or5的抗性遗传及分子标记筛选  

Sunflower Broomrape Resistance Inheritance Analysis and Screening of Molecular Markers

作　　者：张曼[1] 孙墨可 田娟[1] 董玉迪 贾云峰[1] 李慧英[1] 牛庆杰[1] ZHANG Man;SUN Mo-ke;TIAN Juan(Baicheng Academy of Agricultural Science,Baicheng,Jilin 137000)

机构地区：[1]吉林省白城市农业科学院,吉林白城137000

出　　处：《安徽农业科学》2021年第14期89-92,共4页Journal of Anhui Agricultural Sciences

基　　金：国家现代农业产业技术体系和吉林省科学技术厅重点科技研发项目(20180201070NY)。

摘　　要：以向日葵高感列当E的自交系09110-21264162A为母本,与抗病自交系J-09R进行杂交构建F 2群体,分析了向日葵抗列当E的抗性遗传规律,并且采用SSR技术在亲本和F 2代群体中筛选与抗列当基因Or5连锁的分子标记。结果表明:F 2群体的抗感比符合3∶1的分离规律,筛选出与抗列当基因Or5连锁的1个分子标记ORS1036。经F 2分离群体验证,该标记的表型鉴定结果与分子检测结果的一致性为94%。将该标记对84份自交系进行筛选,获得了15份可能携带Or5基因的抗列当资源,为抗性育种提供宝贵资源。The study was carried out by cross-breeding between sunflower broomrape E susceptible inbred line 09110-21264162A and the resistant inbred line J-09R as parents to construct the F 2 population.The parents,F 1 and F 2 generations were inculated with broomrape and the inheritance of resistance to the plant was analyzed according to the results of phenotype observation.The linked marker was screened with two parents and a F 2 population by SSR technology.The results revealed that the identification result of the individual plant of the F 2 population was in line with the anti-infection ratio of 1R:3S.The Molecular Marker,ORS1036,which was linked to Or5,was screened from F 2 population and the parents by SSR technique.The results of phenotypic identification was 94%consistent with the molecular detection.This marker ORS1036 was used to screen 84 inbred lines and 15 materials which contained the marker were obtained,which could provide valuable resources for resistance breeding.

关 键 词：向日葵 抗列当 SSR分子标记 

分 类 号：S565.5[农业科学—作物学]