LC-MS/MS法测定大鼠血浆中EGCG及其药动学研究  

Determination of EGCG in Rat Plasma by LC-MS/MS and Its Pharmacokinetics Study

作　　者：乔妙 朱朋艳 赵云丽 黄艳苹[1,3] 吴凡 高文分 盛军 袁文娟[1,3] 王宣军 QIAO Miao;ZHU Peng-yan;ZHAO Yun-li(Yunnan Agricultural University,Key Laboratory of Pu’er Tea Science,Ministry of Education,Kunming,Yunnan 650201;College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201;College of Science,Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学,普洱茶教育部重点实验室,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南农业大学理学院,云南昆明650201 [4]云南省食品药品监督检验研究院,云南昆明650011

出　　处：《安徽农业科学》2021年第14期172-175,179,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(32060084);云南省应用基础研究项目(2019FB119);国家级大学生创新创业训练计划支持项目(202010676050)。

摘　　要：[目的]建立一种LC-MS/MS测定大鼠血浆中EGCG浓度的方法,进而研究其药动学。[方法]选用Agilent SB-C 18(4.6 mm×250 mm,5μm),乙腈-水(90∶10)为流动相,等度洗脱,ESI离子源,多反应离子监测,负离子扫描;以香草醛为内标,采用内标法定量。[结果]EGCG和内标不受基质干扰;定量限分别为10 ng/mL(EGCG)和5 ng/mL(香草醛),血浆样品批内和批间精密度(RSD)≤11.5%,准确度在92.0%~118.9%,萃取回收率≥92.7%,基质效应为95.3%~115.3%,大鼠腹腔注射20 mg/kg EGCG后的血浆动力学符合二室模型,消除半衰期较长,表观分布容积(Vd)大于总体水体积。[结论]该方法重现性好、分析时间短,能满足EGCG在大鼠血浆中浓度的检测要求,并符合《中国药典》2015年版对生物样品分析的要求,可用于大鼠血浆中EGCG的浓度测定和药代动力学研究。[Objective]To establish a LC-MS/MS method to determine the concentration of EGCG in rat plasma and to study its pharmacokinetics.[Method]Choose Agilent SB-C 18(4.6 mm×250 mm,5μm),acetonitrile-water(90∶10)as mobile phase,isocratic elution,ESI ion source,multi-reactive ion monitoring,negative ion scanning.Vanillin was used as the internal standard,and the internal standard method was used for quantification.[Result]In this method,detection of EGCG and IS were not interfered by the matix,LOQ were 10 ng/mL(EGCG)and 5 ng/mL(vanillin)respectively,the intra-assay and inter-assay precision(RSD)of plasma samples was≤11.5%,the accuracy was 92.0%-118.9%,and the extraction recovery rate≥92.7%,ME was 95.3%-115.3%.After injection of 20 mg/kg EGCG,the plasma dynamics of EGCG was in accordance with the two compartment model,the apparent volume of distribution(Vd)values of EGCG was larger than total body water volume.[Concusion]This method has good reproducibility and short analysis time,can meet the detection requirements of EGCG concentration in rat plasma,and meets the requirements for the analysis of biological samples in the 2015 edition of the Chinese Pharmacopoeia,which can be applied to the pharmacokinetic study of EGCG in rats.

关 键 词：高效液相色谱-质谱联用 EGCG 大鼠血浆 浓度 药代动力学 

分 类 号：R285[医药卫生—中药学]