基于叶片光谱分析的烟草马铃薯Y病毒病严重度诊断  

Diagnosis of tobacco PVY severity based on leaf spectral analysis

作　　者：刘勇昌 耿丽 高强 宋晓欣 王新伟[1] 任广伟[1] LIU Yongchang;GENG Li;GAO Qiang;SONG Xiaoxin;WANG Xinwei;REN Guangwei(Institute of Tobacco Research of CAAS,Qingdao 266101,Shandong,China;Yishui Branch of Shandong Linyi Tobacco Co.,Ltd.,Yishui 276400,Shandong,China;Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]中国农业科学院烟草研究所,山东省青岛市266101 [2]山东临沂烟草有限公司沂水分公司,山东省沂水县276400 [3]河南省农业科学院植物保护研究所,郑州市450002

出　　处：《烟草科技》2021年第7期23-28,共6页Tobacco Science & Technology

基　　金：中国烟草总公司重庆市公司资助项目“重庆烟草病虫害智能预警体系关键技术开发与应用”(B20202NY1336);中国烟草总公司山东省公司资助项目“山东烟草病虫害预测预报与综合防治网络高效运转模式研究与应用”(201508);中国烟草总公司绿色防控重大专项项目“烟草主要叶斑类病害监测预警及绿色防控技术研究与应用”[110202001035(LS-04)]。

摘　　要：为利用光谱分析方法实现马铃薯Y病毒(PVY)病的有效监测预警,采用ASD非成像光谱仪获取了感病后不同严重度烟草叶片的光谱数据。分析结果表明,随着马铃薯Y病毒病严重度的增加,叶片光谱反射率在可见光波段(400~700 nm)内呈现逐渐升高的趋势,在近红外波段(700~1300 nm)内呈现逐渐降低的趋势。利用一元线性回归分析法筛选出与马铃薯Y病毒病严重度呈显著相关的波长,并基于敏感波长453、518、572、708和775 nm的光谱反射率建立了病害严重度逐步回归诊断模型,模型验证决定系数(R^(2))为0.67,均方根误差(RMSE)为1.97,预测准确度为83.84%。因此,烟草马铃薯Y病毒病侵染后不同严重度的烟草在特定波段内的光谱反射率存在显著差异,利用逐步回归法建立预测模型可实现对马铃薯Y病毒病严重度的诊断。To realize effective monitoring and early warning of Potato virus Y(PVY)infection via spectral analysis,leaf spectra from PVY-infected tobacco with different severities were obtained by using ASD non-imaging spectrometer.The analysis results showed that with the increase of PVY severity,the leaf spectral reflectance increased gradually in the visible band(400-700 nm)and decreased gradually in the near-infrared band(700-1300 nm).The two bands significantly correlated with the severity of PVY-infected tobacco were screened by unitary linear recursive analysis.Based on the spectral reflectance at the band combinations of 453,518,572,708 and 775 nm,a stepwise regression diagnosis model for PVY severity grade was established.The verification decision coefficient(R^(2))of the model was 0.67 and its root mean square error(RMSE)was 1.97 with the prediction accuracy of 83.84%.Therefore,the spectral reflectance of tobacco with different severities of PVY infection was significantly different in specific bands.The prediction model established by the stepwise regression method could be used to diagnose PVY severity.

关 键 词：烟草 马铃薯Y病毒(PVY) 光谱反射率 回归模型 严重度 

分 类 号：S432.41[农业科学—植物病理学]