舒芬太尼通过调控miR-410-3p/TIAM1轴影响宫颈癌细胞增殖、迁移和侵袭的机制研究  

Study on the Mechanism of Sufentanil Affecting the Proliferation,Migration and Invasion of Cervical Cancer Cells by Regulating miR-410-3p/TIAM1 Axis

作　　者：赵健衡 张帮健 王丽 蒋燕 Zhao Jianheng;Zhang Bangjian;Wang Li;Jiang Yan(Department of Anesthesiology,Panzhihua Central Hospital,Sichuan Panzhihua 617000,China)

机构地区：[1]攀枝花中心医院麻醉科,四川攀枝花617000

出　　处：《中国药师》2021年第7期267-272,共6页China Pharmacist

摘　　要：目的:探讨舒芬太尼在宫颈癌细胞增殖、迁移和侵袭中的作用与机制。方法:体外培养宫颈癌Hela细胞,用不同剂量(2,10,50 ng·ml^(-1))舒芬太尼干预24 h、转染miR-410-3p mimics或T淋巴瘤侵袭转移诱导因子1(TIAM1)干扰RNA至Hela细胞、或50 ng·ml^(-1)舒芬太尼干预转染miR-410-3p抑制剂的Hela细胞24 h后,细胞计数试剂盒-8(CCK-8)检测细胞存活率,Transwell检测细胞迁移和侵袭,实时荧光定量PCR(RT-qPCR)检测细胞中miR-410-3p表达,蛋白印迹法检测细胞中细胞增殖抗原Ki67、基质金属蛋白酶2(MMP-2)和TIAM1蛋白表达。双荧光素酶报告基因实验验证miR-410-3p与TIAM1的调控关系。结果:舒芬太尼可降低Hela细胞存活率、迁移数、侵袭数及Ki67、MMP-2和TIAM1蛋白表达(P<0.05),促进miR-410-3p表达(P<0.05)。上调miR-410-3p或下调TIAM1后,Hela细胞存活率、迁移数、侵袭数及Ki67和MMP-2蛋白表达均降低(P<0.05)。miR-410-3p靶向负调控TIAM1表达。下调miR-410-3p逆转舒芬太尼对Hela细胞增殖、迁移和侵袭的影响。结论:舒芬太尼可能通过调控miR-410-3p/TIAM1轴降低宫颈癌Hela细胞的增殖、迁移和侵袭能力。Objective:To explore the effect and mechanism of sufentanil on the proliferation,migration and invasion of cervical cancer cells.Methods:Cervical cancer Hela cells were cultured in vitro.After Hela cells were treated with different doses(2,10,50 ng·ml^(-1))of sufentanil for 24 h,or Hela cells were transfected with miR-410-3p mimics or TIAM1 small interfering RNA,or Hela cells transfected with miR-410-3p inhibitor were treated with 50 ng~ml-1 sufentanil for 24 h,CCK-8 was used to detect cell survival rate,Transwell was used to detect cell migration and invasion,RT-qPCR was used to detect the expression of miR-410-3p,and Western blot was used to detect the expressions of Ki67,MMP-2 and TIAM1.The dual luciferase reporter gene experiment was applied to verify the regulatory relationship between miR-410-3p and TIAM1.Results:Sufentanil reduced the survival rate,the number of migration and invasion of Hela cells,and decrease the protein expressions of Ki67,MMP-2 and TIAM1(P<0.05),while promoted the expression of miR-410-3p(P<0.05),and all the effects were dose-dependent.After up-regulating miR-410-3p or down-regulating TIAM1,the survival rate of Hela cells,the number of migration and invasion,and protein expressions of Ki67 and MMP-2 decreased(P<0.05).miR-410-3p could target and negatively regulate the expression of TIAM1.Down-regulating miR-410-3p reversed the effects of sufentanil on the proliferation,migration and invasion of Hela cells.Conclusion:Sufentanil may reduce the proliferation,migration and invasion of cervical cancer Hela cells by regulating the miR-410-3p/TIAM1 axis.

关 键 词：宫颈癌 舒芬太尼 miR-410-3p T淋巴瘤侵袭转移诱导因子1 细胞增殖 迁移 侵袭 

分 类 号：R965.1[医药卫生—药理学]