两相厌氧消化纤维素乙醇发酵残留物及微生物菌群结构分析  

ANALYSIS OF TWO-PHASE ANAEROBIC DIGESTION AND MICROBIAL COMMUNITY STRUCTURE OF CELLULOSIC ETHANOL ETHANOL FERMENTATION STILLAGE

作　　者：陈乐 刘磊[1] 万平[1] 杨秀山[1] 田沈[1] Chen Le;Liu Lei;Wan Ping;Yang Xiushan;Tian Shen(College of Life Sciences,Capital Normal University,Beijing 100048,China)

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《太阳能学报》2021年第6期476-481,共6页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(31971202);国家科技支撑计划(2019YFB1503802)。

摘　　要：为了探究木质纤维素原料乙醇发酵残留物的产甲烷能力,以汽爆预处理狼尾草高底物乙醇发酵全残留物为底物进行两相厌氧消化产甲烷,同时分析其甲烷相微生物菌群结构。结果表明:酸化反应器中主要产物是丁酸和乙酸,纤维素转化率和VS去除率分别为89.0%和85.3%。酸化产物在甲烷相反应器中被有效降解,获得的平均甲烷浓度和平均甲烷产率分别为69.09%和298.5 L/kg COD。Geobacter是产甲烷反应器中的主要细菌,乙酸型产甲烷菌在原始接种物中占优势。然而,随着有机负荷的提升,氢营养型产甲烷菌的富集优势逐渐明显,有利于挥发酸进一步降解产甲烷从而提高厌氧消化性能。To study the methanogenic characteristics of lignocellulosic ethanol fermentation stillage,In this study,the performance for the two-phase AD with ethanol fermentation stillage of steam explosion pretreatment Pennisetum purpureum as the substrate was investigated and the microbial community structure of methanogenic phase was also analyzed.The results show that the main fermentation products in the acidification reactor are butyric acid and acetic acid,and the cellulose conversion rate and VS removal rate is 89%and 85.3%respectively.The acidification products are effectively degraded in the methanogenic reactor and the average methane concentration and average methane yield obtained is 69.09%and 298.5 L/Kg COD respectively.Geobacter is the major bacteria in methanogenic reactor while acetoclastic methanogens is dominant in the original inoculation.However hydrogenotrophic methanogens increase with the improvement of organic load rate,which is conducive to the further degradation of volatile fatty acids and improves the anaerobic digestion performance.

关 键 词：纤维素乙醇 发酵残留物 厌氧消化 菌群结构 甲烷 

分 类 号：TK513.5[动力工程及工程热物理—热能工程]