添加胃肠肽的牛乳对食物过敏和食物不耐受的调节作用  

Regulatory effects of cow milk added with gastrointestinal peptides on food allergy and food intolerance

作　　者：万雪梦 谢咏梅 汪志凌 高珊 王丽媛 万朝敏 Wan Xuemeng;Xie Yongmei;Wang Zhiling;Gao Shan;Wang Liyuan;Wan Chaomin(Department of Pediatrics,West China Second University Hospital of Sichuan University Key Laboratory of Birth Defects and Related Diseases of Women and Children Ministry of Education,Chengdu 610041,China;Graduate Educational Administration of West China Medical College,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西第二医院儿科,出生缺陷与相关妇儿疾病教育部重点实验室,成都610041 [2]四川大学华西临床医学院研究生部,成都610041

出　　处：《中华实用儿科临床杂志》2021年第13期1023-1026,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：四川省科技厅重点研发项目(2019YFG0165);四川大学华西第二医院临床科研基金(KL013)。

摘　　要：目的在牛乳中添加等同母乳剂量的生长抑素(SST)及胃动素(MTL),探讨其对食物过敏/不耐受的调节作用。方法挪威大鼠(BN)幼鼠给予母乳(母乳组)、牛乳(牛乳组)、添加SST的牛乳(SST组)、添加MTL的牛乳(MTL组)、添加SST+MTL的牛乳(SST+MTL组)5种方式喂养4周,并配合皮肤致敏。每周量化评定临床损害。酶联免疫吸附法(ELISA)测定血总免疫球蛋白E(IgE)及粪钙卫蛋白(FC)水平。葡聚糖蓝法测定胃排空比率及小肠推进比。结果母乳组、牛乳组、SST组、MTL组及SST+MTL组的总IgE水平分别为(45.75±5.05)μg/L、(580.42±45.24)μg/L、(290.38±22.88)μg/L、(424.26±22.17)μg/L、(209.49±17.59)μg/L;FC水平分别为(149.07±24.78)μg/g、(458.85±33.81)μg/g、(343.63±34.97)μg/g、(407.79±29.62)μg/g、(296.83±28.77)μg/g;第4周临床损害总评分分别为:(0.50±0.61)分、(9.37±1.04)分、(6.83±1.49)分、(7.00±1.14)分、(5.37±1.19)分。与牛乳组比较,SST组、MTL组及SST+MTL组的IgE、FC水平及临床损害评分均显著降低,差异均有统计学意义(均P<0.01)。牛乳组临床损害最重,SST+MTL组临床损害最轻,SST+MTL组最接近母乳喂养天然状态。MTL组的胃排空比率最接近母乳组[(92.52±6.27)%比(100.00±9.70)%,P<0.05]。牛乳组有明显腹泻及小肠推进比过快,小肠推进比母乳组为(39.32±2.61)%,牛乳组为(71.96±4.43)%,2组比较差异有统计学意义(P<0.01)。SST+MTL组的肠动力有所下降,但这刚好对抗了牛乳过敏产生的腹泻,SST+MTL组小肠推进比为(38.90±2.65)%,母乳组为(39.32±2.61)%,2组比较差异无统计学意义(P>0.05)。结论牛乳中添加母乳剂量的SST及MTL使牛乳的致敏性及胃肠耐受性更接近母乳,SST有利于抑制过敏免疫损伤,MTL有利于改善牛乳的胃肠耐受性,且SST与MTL有拮抗平衡调节作用,二者同时添加,可协同提高牛乳的胃肠耐受性。Objective To explore the regulatory effects of cow milk with the addition of breast milk equivalent dose of somatostatin(SST)and motilin(MTL)on food allergy and food intolerance.Methods Young Brown Norway(BN)rats were divided into 5 groups and fed with pure breast milk(breast milk group),cow milk(cow milk group),cow milk added with SST(SST group),cow milk added with MTL(MTL group)and cow milk added with both SST+MTL(SST+MTL group).Allergic irritation was enhanced with skin smear at the same time.Clinical damages were quantified weekly.Levels of serous total Immunoglobulin E(IgE)and fecal calprotectin(FC)were detected by the enzyme-linked immunosorbent assay(ELISA).Gastric emptying ratio and intestinal propulsion ratio were measured by method of dextran blue.Results In breast milk group,cow milk group,SST group,MTL group and SST+MTL group,the levels of IgE were(45.75±5.05)μg/L,(580.42±45.24)μg/L,(290.38±22.88)μg/L,(424.26±22.17)μg/L,(209.49±17.59)μg/L,respectively;FC level were(149.07±24.78)μg/g,(458.85±33.81)μg/g,(343.63±34.97)μg/g,(407.79±29.62)μg/g,(296.83±28.77)μg/g,respectively;the total score of clinical damage were(0.50±0.61)scores,(9.37±1.04)scores,(6.83±1.49)scores,(7.00±1.14)scores,(5.37±1.19)scores,respectively.The cow milk group had the highest scores of clinical damages.Compared with the cow milk group,the clinical damage score,IgE and FC of the SST,MTL and SST+MTL groups had significantly lower levels,and there was significant difference among them(all P<0.01).The general status of the SST+MTL group was most similar to the breast milk group.The gastric emptying rate of MTL group was the closest to that of breast milk group[(92.52±6.27)%vs.(100.00±9.70)%,P<0.05].There were obvious diarrhea and fast small intestinal propulsion in cow milk group,the small intestinal propulsion ratio in breast milk group was(39.32±2.61)%,and(71.96±4.43)%in cow milk group,the difference was statistically significant between the 2 groups(P<0.01).The intestinal motility of SST+MTL group was decr

关 键 词：生长抑素 胃动素 食物过敏 食物不耐受 钙卫蛋白 大鼠 

分 类 号：R725.9[医药卫生—儿科]