An improved method for whole-mount preparation of rat myenteric plexus and its catecholaminergic innervation  

作　　者：Zhou Li Li Yong Zhang Yang Wang Zhiyong 

机构地区：[1]Department of Human Anatomy,Xinxiang Medical University,Xinxiang 453003

出　　处：《解剖学杂志》2021年第S01期260-260,共1页Chinese Journal of Anatomy

摘　　要：We present an optimal and reproducible protocol by which the whole mount myenteric plexus(MP)preparation of adult rat stomach and intestine can be isolated with integrity.We also investigate whether the rat MP contains intrinsic catecholaminergic neurons.Critical steps of the whole mount preparation were optimized,including tissue clearing,fixation,size,placement and the detailed dissection procedures.Additionally,whole mount fluorescent immunohistochemistry of tyrosine hydroxylase(TH),the rate-limiting enzyme of catecholaminergic biosynthesis was used as aneuronal marker to ascertain the existence of intrinsic catecholaminergic neurons.

关 键 词：MOUNT PREPARATION INTRINSIC 

分 类 号：O17[理学—数学]